Kim Ryunhee, Osako Yutaro, Yamane Hisayo, Tao Ryutaro, Miyagawa Hisashi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Biosci Biotechnol Biochem. 2021 Feb 24;85(3):467-475. doi: 10.1093/bbb/zbaa083.
To investigate the modulation of endogenous indole-3-acetic acid (IAA) level by biosynthesis and inactivation during floral development, IAA and its metabolites were analyzed by LC-ESI/MS/MS in Lychee (Litchi chinensis Sonn.) flowers. In the bloomed flowers, the level of free IAA was higher in males than in females. In contrast, the total sum level of IAA metabolites was higher in females than in males, suggesting a higher biosynthetic activity of IAA in the females before the bloom. A detailed time-course analysis from the bud stage to the developing flower stage showed higher levels of IAA in females than males. The major metabolites were oxidized IAA in both sexes. The results suggest that IAA is involved in the maturation of female floral tissues in lychee, and oxidative metabolism plays an essential role in controlling the free IAA levels therein.
为研究在花发育过程中生物合成和失活对内源吲哚 - 3 - 乙酸（IAA）水平的调节作用，采用液相色谱 - 电喷雾串联质谱法（LC - ESI/MS/MS）分析了荔枝（Litchi chinensis Sonn.）花中的IAA及其代谢产物。在盛开的花朵中，雄性花中游离IAA的水平高于雌性花。相反，IAA代谢产物的总量在雌性花中高于雄性花，这表明在开花前雌性花中IAA的生物合成活性更高。从芽期到发育中的花期的详细时间进程分析表明，雌性花中的IAA水平高于雄性花。两性中的主要代谢产物均为氧化型IAA。结果表明，IAA参与荔枝雌性花组织的成熟，氧化代谢在控制其中游离IAA水平方面起着重要作用。
