Aleksandrova E N, Nasonov E L, Vinogradov V A, Titov M I
Biull Eksp Biol Med. 1988 Apr;105(4):442-5.
The effect of synthetic opioid peptide analogue dalargin on the natural cytotoxicity of lymphocytes from the peripheral human blood has been studied in respect to target cells K-562. Natural cytotoxicity was determined by assessing the cytotoxicity of individual effector cells in agarose. It has been shown that preincubation of lymphocytes with 10(-14)--10(-8) M of dalargin enhances the binding of NK-cells with targets and the formation of conjugates with killed targets and increases the percentage of active NK-cells. In some cases bimodal kinetics of a stimulating dalargin effect on the natural cytotoxicity has been observed, with two peaks of the peptide activity being in the range of 10(14)--10(-12) and 10(-10)--10(-6) M. Naloxone at a concentration of 10(-6) M acted as an agonist of opioid receptors, enhancing the formation of effector-target conjugates and NK-cell lysis abilities. A stimulating effect of dalargin on the natural cytotoxicity was more marked than that of recombinant human alpha-interferon. The possible use of dalargin in pathological conditions characterized by disturbed NK-cell function is discussed.
就靶细胞K-562而言，已研究了合成阿片样肽类似物达拉根对人外周血淋巴细胞自然细胞毒性的影响。通过评估琼脂糖中单个效应细胞的细胞毒性来测定自然细胞毒性。结果表明，淋巴细胞与10(-14) - 10(-8)M的达拉根预孵育可增强NK细胞与靶标的结合以及与被杀伤靶标形成共轭物，并增加活性NK细胞的百分比。在某些情况下，观察到达拉根对自然细胞毒性的刺激作用具有双峰动力学，肽活性的两个峰值在10(-14) - 10(-12)和10(-10) - 10(-6)M范围内。浓度为10(-6)M的纳洛酮作为阿片受体的激动剂，增强了效应器-靶标共轭物的形成和NK细胞的裂解能力。达拉根对自然细胞毒性的刺激作用比重组人α干扰素更显著。文中讨论了达拉根在以NK细胞功能紊乱为特征的病理状况中的可能用途。
