Department of Geriatrics and Gerontology, National Taiwan University Hospital, Taipei, Taiwan.
Department of Family Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Eur J Clin Invest. 2021 Jul;51(7):e13522. doi: 10.1111/eci.13522. Epub 2021 Mar 16.
Inflammation plays an important role in the ageing process in which monocytes/macrophages are important players. Intercellular adhesion molecule-1 (ICAM-1), tumour necrosis factor-α (TNF-α) and Toll-like receptor 4 (TLR4) are well-known inflammatory markers. This study aimed to investigate the relationship between age and the expression and correlation of ICAM-1, TNF-α and TLR4 mRNA in peripheral blood mononuclear cells (PBMCs).
A total of 239 participants were recruited in a medical centre in Taiwan. The mRNA isolated from the PBMCs was used to determine the levels of ICAM-1, TNF-α and TLR4 mRNAs with real-time polymerase chain reaction (PCR). The propensity-matched analysis was also applied for subgroup analysis.
When compared 189 older adults (≧65 years) to 50 younger adults (<65 years), the ICAM-1, TNF-α and TLR4 mRNA levels in PBMCs were significantly higher in older adults (2.00 ± 0.72 vs 0.87 ± 0.34 for ICAM-1, 2.32 ± 0.69 vs 1.15 ± 0.44 for TNF-α and 1.56 ± 0.47 vs 1.05 ± 0.51 for TLR4, and all P < .0001). Also, both age and TLR4 were independent factors affecting mononuclear cell ICAM-1 in the multiple linear regression analysis (P < .0001).
The mRNA levels of ICAM-1 and TLR4 in PBMCs are higher in older adults than those in younger adults. TLR4 is an independent factor affecting ICAM-1 expression in PBMCs, especially in older adults. This may suggest that ICAM-1 and TLR4 in PBMCs are potential biomarkers and their relationship may shed some light on the ageing process.
炎症在衰老过程中起着重要作用，其中单核细胞/巨噬细胞是重要的参与者。细胞间黏附分子-1（ICAM-1）、肿瘤坏死因子-α（TNF-α）和 Toll 样受体 4（TLR4）是众所周知的炎症标志物。本研究旨在探讨外周血单个核细胞（PBMC）中 ICAM-1、TNF-α 和 TLR4 mRNA 的表达与相关性与年龄的关系。
在台湾的一家医疗中心招募了 239 名参与者。使用实时聚合酶链反应（PCR）从 PBMC 中分离的 mRNA 来确定 ICAM-1、TNF-α 和 TLR4 mRNA 的水平。还应用倾向评分匹配分析进行亚组分析。
与 50 名年龄较小的成年人（<65 岁）相比，189 名年龄较大的成年人（≧65 岁）的 PBMC 中 ICAM-1、TNF-α 和 TLR4 mRNA 水平显著升高（2.00±0.72 对 0.87±0.34 用于 ICAM-1，2.32±0.69 对 1.15±0.44 用于 TNF-α，1.56±0.47 对 1.05±0.51 用于 TLR4，均 P<.0001）。此外，在多元线性回归分析中，年龄和 TLR4 均为单核细胞 ICAM-1 的独立影响因素（P<.0001）。
与年轻成年人相比，老年成年人 PBMC 中 ICAM-1 和 TLR4 的 mRNA 水平更高。TLR4 是影响 PBMC 中 ICAM-1 表达的独立因素，尤其是在老年人中。这可能表明 PBMC 中的 ICAM-1 和 TLR4 是潜在的生物标志物，它们的关系可能揭示衰老过程的一些线索。
