Busija D W, Wagerle L C, Pourcyrous M, Leffler C W
Department of Physiology and Biophysics, University of Tennessee, Memphis 38163.
Brain Res. 1988 Jan 26;439(1-2):122-6. doi: 10.1016/0006-8993(88)91468-0.
We investigated effects of exogenous acetylcholine on prostanoid synthesis by parietal cortex in neonatal pigs. Cerebrospinal fluid (CSF) with no drug, and CSF containing acetylcholine at 10(-6) to 10(-3) M was injected under a 'closed' cranial window, and after 5 min the CSF was collected and analyzed by radioimmunoassay for prostaglandin (PG) E2, PGF2 alpha, PGD2, 6-keto-PGF1 alpha (the hydrolysis product of prostacyclin), and thromboxane (TX) B2 (the hydrolysis product of TXA2). PGE2 and PGF2 alpha were the predominant prostanoids in CSF under control conditions. Levels of all CSF prostanoids increased after topical application of acetylcholine, with the largest increases being for PGE2 and PGF2 alpha. During control conditions, levels were 1294 +/- 170 (mean +/- S.E.M.) pg/ml for PGE2 (n = 16), 1032 +/- 143 pg/ml for PGF2 alpha (n = 3), 659 +/- 92 pg/ml for 6-keto-PGF1 alpha (n = 15), 141 +/- 44 pg/ml for TXB2 (n = 12), and were below detectable levels for PGD2. Following application of 10(-3) M acetylcholine, levels were 34,535 +/- 5438 pg/ml for PGE2, 15,539 +/- 2772 pg/ml for PGF2 alpha, 2967 +/- 547 pg/ml for 6-keto-PGF1 alpha, 580 +/- 105 pg/ml for TXB2, and 556 +/- 221 pg/ml for PGD2. These results suggest that prostanoids could play a role in mediating effects of acetylcholine in the brain, or in modulating acetylcholine release via a negative feedback mechanism.
我们研究了外源性乙酰胆碱对新生猪顶叶皮质前列腺素合成的影响。在“封闭”的颅窗下注射不含药物的脑脊液（CSF）以及含有10⁻⁶至10⁻³M乙酰胆碱的脑脊液，5分钟后收集脑脊液，并通过放射免疫分析法分析前列腺素（PG）E2、PGF2α、PGD2、6-酮-PGF1α（前列环素的水解产物）和血栓素（TX）B2（TXA2的水解产物）。在对照条件下，PGE2和PGF2α是脑脊液中主要的前列腺素。局部应用乙酰胆碱后，所有脑脊液前列腺素水平均升高，其中PGE2和PGF2α升高幅度最大。在对照条件下，PGE2水平为1294±170（平均值±标准误）pg/ml（n = 16），PGF2α为1032±143 pg/ml（n = 3），6-酮-PGF1α为659±92 pg/ml（n = 15），TXB2为141±44 pg/ml（n = 12），PGD2低于可检测水平。应用10⁻³M乙酰胆碱后，PGE2水平为34535±5438 pg/ml，PGF2α为15539±2772 pg/ml，6-酮-PGF1α为2967±547 pg/ml，TXB2为580±105 pg/ml，PGD2为556±221 pg/ml。这些结果表明，前列腺素可能在介导大脑中乙酰胆碱的作用或通过负反馈机制调节乙酰胆碱释放中发挥作用。
