Chmielowska J, Stewart M G, Bourne R C
Brain Research Group, Open University, Milton Keynes, U.K.
Brain Res. 1988 Jan 26;439(1-2):155-68. doi: 10.1016/0006-8993(88)91472-2.
The location and morphological characteristics of gamma-aminobutyric acid (GABA)-immunopositive cells and their processes were studied in rat and mouse first somatosensory (SI) cortex (including 'barrels') in layer IV, and layers above (I-III), and below (V and VI). In coronal sections of SI cortex of both species GABA-immunopositive cells and punctate profiles were found in each of layers I-VI. The cells were of various sizes; the largest, located in layers III and V of each species, resemble the large basket cells seen in Golgi-impregnated material. Most of the immunopositive cells were multipolar and circular or ellipsoidal in shape, but occasionally bipolar cells with fusiform perikarya were also seen. In coronal sections, immunopositive cells did not form a characteristic pattern. GABA-immunopositive cells were observed to be most numerous in the supragranular layers whereas GABA-positive punctate profiles were more numerous in layer IV. In tangential sections from layer IV of SI cortex of both species, GABA-immunopositive cells, processes and punctate profiles were visible throughout the entire barrel field. The pattern of distribution of immunopositive cells was similar (a) in two different morphological groups--i.e. the posteromedial barrel subfield (PMBSF) and the anterolateral barrel subfield (ALBSF) in rat barrel field, and (b) in PMBSF barrels of both rat and mouse (excluding differences due to structural dissimilarities between rat and mouse barrels). GABA-immunopositive neurons were grouped mainly in the barrel side and septum and were visible frequently in small clusters. In barrels of both species GABA-immunopositive cells were of a variety of sizes and ranged in shape from ellipsoidal to circular.
在大鼠和小鼠的第一体感（SI）皮层（包括IV层的“桶状结构”）以及IV层上方（I - III层）和下方（V层和VI层），研究了γ-氨基丁酸（GABA）免疫阳性细胞及其突起的位置和形态特征。在这两个物种的SI皮层冠状切片中，I - VI层均发现了GABA免疫阳性细胞和点状分布。细胞大小各异；每个物种III层和V层中最大的细胞类似于在高尔基染色材料中看到的大篮状细胞。大多数免疫阳性细胞是多极的，呈圆形或椭圆形，但偶尔也可见到具有梭形胞体的双极细胞。在冠状切片中，免疫阳性细胞未形成特征性模式。观察到GABA免疫阳性细胞在颗粒上层中数量最多，而GABA阳性点状分布在IV层中更多。在这两个物种SI皮层IV层的切线切片中，整个桶状区都可见到GABA免疫阳性细胞、突起和点状分布。免疫阳性细胞的分布模式在以下两种情况中相似：（a）在大鼠桶状区的两个不同形态学组中，即后内侧桶状亚区（PMBSF）和前外侧桶状亚区（ALBSF）；（b）在大鼠和小鼠的PMBSF桶状结构中（排除大鼠和小鼠桶状结构之间结构差异导致的不同）。GABA免疫阳性神经元主要聚集在桶状结构的边缘和间隔处，且经常可见小簇状分布。在这两个物种的桶状结构中，GABA免疫阳性细胞大小不一，形状从椭圆形到圆形不等。
