Kossut M, Stewart M G, Siucinska E, Bourne R C, Gabbott P L
Nencki Institute of Experimental Biology, Warsaw, Poland.
Brain Res. 1991 Jan 4;538(1):165-70. doi: 10.1016/0006-8993(91)90393-a.
Whisker follicles were surgically ablated (lesioned) on two entire rows (B and C) of the left snout of two groups of Swiss mice, in the first 2 days after birth, and in the second group at 8 weeks of age (adults). Two months after surgery GABA-immunoreactivity (GABA-IR) was examined in tangential sections of the first somatosensory (SI) 'barrel' cortex of these two groups (at which time it was also confirmed that the follicles had not regrown). In the adult-lesioned mice all of the barrels appeared intact and visually similar in both the experimental (right) and control (left) hemispheres. The staining pattern of immunopositive cells and puncta was qualitatively similar to that which we have described previously. However, in the neonatally-lesioned mice barrel rows B and C were not visible in the right hemisphere sections and there was a marked reduction in GABA-IR, with fewer immunopositive cells. Many of those that did show GABA-IR stained only weakly and the puncta were also fewer in number than in the left (control) hemisphere where the GABA-IR pattern and staining intensity was normal.
在出生后的头两天，对两组瑞士小鼠左鼻的两整排（B和C）触须毛囊进行手术切除（损伤）；对第二组小鼠在8周龄（成年）时进行同样操作。术后两个月，在这两组小鼠第一体感（SI）“桶状”皮质的切线切片中检测γ-氨基丁酸免疫反应性（GABA-IR）（此时也证实毛囊未再生）。在成年期损伤的小鼠中，所有的桶状结构在实验（右侧）和对照（左侧）半球均完好无损且外观相似。免疫阳性细胞和斑点的染色模式在质量上与我们之前描述的相似。然而，在新生期损伤的小鼠中，右侧半球切片中看不到B和C排桶状结构，GABA-IR明显减少，免疫阳性细胞较少。许多显示GABA-IR的细胞染色较弱，斑点数量也比左侧（对照）半球少，左侧半球的GABA-IR模式和染色强度正常。
