School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China; Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, Anhui, 230601, China; Institute of Physical Science, Institute of Information Technology, Anhui University, Hefei, Anhui, 230601, China.
School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China; Key Laboratory of Human Microenvironment and Precision Medicine of Anhui Higher Education Institutes, Anhui University, Hefei, Anhui, 230601, China.
Biochem Biophys Res Commun. 2021 Apr 2;547:59-64. doi: 10.1016/j.bbrc.2021.02.022. Epub 2021 Feb 14.
Akkermansia muciniphila is a kind of beneficial microorganism colonized in the human gut. A. muciniphila is closely related to human intestinal health and has a good effect on diseases related to intestinal metabolism. The proteins encoded by the Amuc_1098-Amuc_1102 gene cluster, which are related to the formation and assembly of the pilus, are highly expressed in the membrane protein components of A. muciniphila. In this paper, we report the crystal structure of Amuc_1102 at a resolution of 1.75 Å, which adopts an immunoglobulin (Ig)-like fold. Amuc_1102 shares a similar fold to three archaeal proteins related to type IV pilus (T4P)-like structure, Pilin, FlaF, and FlaG, indicating a similar function. Amuc_1102 exists as a trimer both in the crystal structure and in solution, which differs from the assemblies of Pilin, FlaF, and FlaG. This study provides a structural basis for the elucidation of the T4P formation of A. muciniphila.
黏蛋白阿克曼菌是定植于人体肠道的有益微生物。黏蛋白阿克曼菌与人类肠道健康密切相关，对与肠道代谢相关的疾病有良好的作用。与菌毛形成和组装相关的 Amuc_1098-Amuc_1102 基因簇所编码的蛋白质在黏蛋白阿克曼菌的膜蛋白成分中高度表达。在本文中，我们报道了 Amuc_1102 的晶体结构，分辨率为 1.75Å，采用免疫球蛋白（Ig）样折叠。Amuc_1102 与三个与 IV 型菌毛（T4P）样结构相关的古菌蛋白（Pilin、FlaF 和 FlaG）具有相似的折叠，表明具有相似的功能。Amuc_1102 无论是在晶体结构中还是在溶液中均以三聚体形式存在，这与 Pilin、FlaF 和 FlaG 的组装不同。本研究为阐明黏蛋白阿克曼菌的 T4P 形成提供了结构基础。
