Mou Luqiu, Peng Xi, Chen Yan, Xiao Qingjie, Liao Huijuan, Liu Mingfeng, Guo Li, Liu Yang, Zhang Xiaohu, Deng Dong
Division of Obstetrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE, State Key Laboratory of Biotherapy, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
Sichuan University-The Chinese University of Hong Kong Joint Laboratory for Reproductive Medicine, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
Acta Crystallogr F Struct Biol Commun. 2020 Apr 1;76(Pt 4):168-174. doi: 10.1107/S2053230X20004124.
Many human diseases, such as obesity and diabetes, show annual increases in prevalence and often involve intestinal microbes. One such probiotic bacterium, Akkermansia muciniphila, which was discovered a decade ago, has been reported to influence glucose homeostasis and to contribute to gut health. Amuc_1100, a functionally uncharacterized protein of A. muciniphila, was found to be a key active component in reducing the body weight of mice. Here, the crystal structure of Amuc_1100 (residues 31-317), referred to as Amuc_1100*, is reported at 2.1 Å resolution. Amuc_1100* has a similar fold to three proteins related to pilus formation, PilO, PilN and EpsL, indicating a similar function. Biochemical investigations further confirmed a monomeric state for the soluble region of Amuc_1100, which differs from the dimeric states of PilO, PilN and EpsL. This study provides a structural basis for the elucidation of the molecular mechanism of Amuc_1100.
许多人类疾病，如肥胖症和糖尿病，其患病率呈逐年上升趋势，且往往与肠道微生物有关。一种这样的益生菌——嗜黏蛋白阿克曼氏菌，于十年前被发现，据报道它会影响葡萄糖稳态并有助于肠道健康。嗜黏蛋白阿克曼氏菌的一种功能未明的蛋白质Amuc_1100，被发现是减轻小鼠体重的关键活性成分。在此，报道了Amuc_1100（31 - 317位残基）的晶体结构，称为Amuc_1100*，分辨率为2.1 Å。Amuc_1100*与三种与菌毛形成相关的蛋白质PilO、PilN和EpsL具有相似的折叠结构，表明其功能相似。生化研究进一步证实了Amuc_1100可溶性区域的单体状态，这与PilO、PilN和EpsL的二聚体状态不同。本研究为阐明Amuc_1100的分子机制提供了结构基础。
