Rita N, Mursyidah A K, Khadijah S
School of Food Science and Technology, Universiti Malaysia Terengganu, 21030, Kuala Terengganu, Malaysia.
Trop Biomed. 2017 Jun 1;34(2):324-331.
A study was conducted to determine the prevalence of helminthiasis among cattle in seven districts of Terengganu, namely: Kuala Terengganu, Hulu Terengganu, Setiu, Dungun, Kemaman, Marang and Besut. The periods of samplings were from March 2015 to January 2016 conducted between 0800-1400h. A total of 219 faecal samples and 214 blood samples were collected from the animals. The faecal samples were analyzed by faecal sedimentation method, modified McMaster method, culture techniques and identification of third stage larvae morphologically. The blood samples were centrifuged for sera that were used for serological test. Results showed that the overall helminthiasis was made of 55.0% trematodes and 14.0% nematode infection. Out of 55.0% of trematode infection, 41.0% was liver fluke infection, 8.0% was rumen fluke infection and 52.0% was co-infection of liver fluke and rumen fluke. A significant difference in the distribution of trematode eggs was recorded among the animals, χ(2, N=120) = 41, p<0.05. Faecal egg count (FEC) for gastrointestinal (GI) nematodes were ranged from 0 - 800 e.p.g. The mean faecal egg count was 21.5 ± 4.5 e.p.g. Faecal culture revealed that the most prevalent nematodes identified was Haemonchus (81.0%), followed by Trichostrongylus (15.0%) and Oesophagostomum (4.0%), χ(2, N=100) = 104, p<0.05. Sandwich ELISA test revealed 82.0% of the sero samples were positive for liver fluke infection. It can be concluded that trematode infection is an on-going problem in cattle located in Terengganu, which can lead to economic losses for the farmers.
开展了一项研究，以确定登嘉楼七个地区（即瓜拉丁加奴、 Hulu 登嘉楼、仕林河、龙运、甘马挽、马兰和贝苏特）牛群中蠕虫病的流行情况。采样时间为2015年3月至2016年1月，在08:00 - 14:00之间进行。共从动物身上采集了219份粪便样本和214份血液样本。粪便样本通过粪便沉淀法、改良麦克马斯特法、培养技术以及第三期幼虫形态学鉴定进行分析。血液样本离心后获取血清用于血清学检测。结果显示，总体蠕虫病中吸虫感染占55.0%，线虫感染占14.0%。在55.0%的吸虫感染中，41.0%为肝吸虫感染，8.0%为瘤胃吸虫感染，52.0%为肝吸虫和瘤胃吸虫混合感染。动物之间吸虫卵分布存在显著差异，χ(2, N = 120) = 41，p < 0.05。胃肠道线虫的粪便虫卵计数（FEC）范围为0 - 800个虫卵/克粪便。平均粪便虫卵计数为21.5 ± 4.5个虫卵/克粪便。粪便培养显示，鉴定出的最常见线虫是血矛线虫（81.0%），其次是毛圆线虫（15.0%）和食道口线虫（4.0%），χ(2, N = 100) = 104，p < 0.05。夹心ELISA检测显示，82.0%的血清样本肝吸虫感染呈阳性。可以得出结论，吸虫感染是登嘉楼牛群中持续存在的问题，可能给农民带来经济损失。
