Dang Z, Fu Y, Duo H, Fan H, Qiao Z, Guo Z, Feng K, Chui W, Shen X, Geng Qiu J, Ni M, He S, Zhao H, Peng M, Xiao N, Nonaka N, Nasu T, Huang F, Oku Y, Hayashimoto N, Hu W, Li W
Key Laboratory on Biology of Parasite and Vector, Ministry of Health, Shanghai, China; National Center for International Research on Tropical Diseases, Shanghai, China; WHO Collaborating Center for Tropical Diseases, Shanghai, China; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
Academy of Animal and Veterinary Medicine, Qinghai University of Animal and Veterinary Sciences, Xining 810016, China.
Trop Biomed. 2017 Jun 1;34(2):483-490.
In order to understand the epidemiological status of alveolar and cystic echinococcosis in intermediate and definitive hosts in Qinghai Province, China, during the period 2007-2011, we investigated the infection in humans and animals, including yaks, Tibetan sheep, Tibetan dogs, and wild foxes distributed in different counties around the province. Sera from local residents were examined using a rapid serodiagnostic kit to detect specific antibodies against Echinococcus. Seropositive samples were confirmed with B-scan ultrasonography and X-ray examinations. Yaks and Tibetan sheep were checked at slaughterhouses, and cysts and suspicious lesions were collected for analysis. A rapid diagnostic strip was used to detect Echinococcus adults in Tibetan dogs. Positive dogs were dewormed and the parasites collected. Wild foxes were trapped and necropsies performed with particular attention to the intestine. Forty-eight of 735 (6.4%) humans tested were positive and 475 of 854 (55.6%) Tibetan sheep and 85 of 352 (24.15%) yaks were infected with Echinococcus. Across different counties, 214 of 948 (22.57%) Tibetan dogs were positive, and five of 36 (13.9%) wild foxes were infected with Echinococcus. Molecular studies showed that all the infections detected in humans, domestic yaks, and Tibetan sheep were the G1 genotype (E. granulosus), whereas the parasites from Tibetan foxes and Tibetan dogs were E. shiquicus and E. multilocularis, respectively. In conclusion, Echinococcosis is hyperendemic in Qinghai Province in both its intermediate and definitive hosts and the G1 genotype of cystic Echinococcus is the dominant strain.
为了解2007 - 2011年期间中国青海省中间宿主和终末宿主中泡型和囊型棘球蚴病的流行病学状况，我们调查了该省不同县分布的人类和动物（包括牦牛、藏绵羊、藏狗和野生狐狸）的感染情况。使用快速血清诊断试剂盒检测当地居民血清中针对棘球蚴的特异性抗体。血清阳性样本通过B超和X线检查进行确诊。在屠宰场检查牦牛和藏绵羊，收集囊肿和可疑病变进行分析。使用快速诊断试纸条检测藏狗体内的棘球绦虫成虫。对阳性犬进行驱虫并收集寄生虫。诱捕野生狐狸并进行尸检，特别关注肠道。735名受检者中有48人（6.4%）呈阳性，854只藏绵羊中有475只（55.6%）、352头牦牛中有85头（24.15%）感染了棘球蚴。在不同县，948只藏狗中有214只（22.57%）呈阳性，36只野生狐狸中有5只（13.9%）感染了棘球蚴。分子研究表明，在人类、家养牦牛和藏绵羊中检测到的所有感染均为G1基因型（细粒棘球绦虫），而来自藏狐和藏狗的寄生虫分别为石渠棘球绦虫和多房棘球绦虫。总之，棘球蚴病在青海省的中间宿主和终末宿主中均为高度流行，囊型棘球蚴的G1基因型是优势菌株。
