Department of Mental and Physic Health and Preventive Medicine, Pathology Unit, University of Campania Luigi Vanvitelli, Napoli, Italy.
Department of Pathology, University of Verona, Verona, Italy.
Expert Rev Mol Diagn. 2021 Feb;21(2):161-173. doi: 10.1080/14737159.2021.1887733. Epub 2021 Feb 22.
: Multitarget FISH (mFISH) is a technique allowing for simultaneous detection of multiple targets sequences on the same slide through the choice of spectrally distinct fluorophore labels. The mFISH could represent a useful tool in the field of precision oncology.: This review discusses the potential applications of mFISH technology in the molecular diagnosis of different solid and hematological tumors, including non-small cell lung cancers, melanomas, renal cell carcinomas, bladder carcinomas, germ cell tumors, and multiple myeloma, as commonly required in the clinical practice.: In this emerging era of the tailored therapies and newer histo-molecular classifications, there are increasing numbers of predictive and diagnostic biomarkers required for effective clinical care. The mFISH approach may have several applications in the common clinical practice, improving the molecular diagnosis in terms of time, cost and preservation of biomaterial for tumors with a limited amount of tumor available. The mFISH provides several advantages compared to other high-throughput technologies; however, it requires high level of expertise required to interpret complex results.
多靶点荧光原位杂交（mFISH）是一种技术，通过选择光谱上明显不同的荧光染料标记，可以在同一张载玻片上同时检测多个靶序列。mFISH 在精准肿瘤学领域可能是一种有用的工具。
本文讨论了 mFISH 技术在不同实体瘤和血液系统肿瘤的分子诊断中的潜在应用，包括非小细胞肺癌、黑色素瘤、肾细胞癌、膀胱癌、生殖细胞肿瘤和多发性骨髓瘤，这些在临床实践中通常是必需的。
在这个精准治疗和新的组织分子分类的新兴时代，需要越来越多的预测和诊断生物标志物来进行有效的临床护理。mFISH 方法在常见的临床实践中有多种应用，可以在时间、成本和有限肿瘤量的肿瘤生物材料保存方面提高分子诊断的效果。与其他高通量技术相比，mFISH 具有许多优势；然而，它需要高水平的专业知识来解释复杂的结果。
