Cannella M S, Roisen F J, Ogawa T, Sugimoto M, Ledeen R W
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
Brain Res. 1988 Mar 1;467(1):137-43. doi: 10.1016/0165-3806(88)90075-2.
A variety of naturally occurring ganglioside structures were previously shown to be effective agents for inducing neurite outgrowth of primary neurons and neuroblastoma lines. We report here the results of similar experiments with a synthetic epimer of GM3 (epi-GM3) possessing a neuraminidase-resistant beta-ketosidic linkage. This substance was found to enhance neuritogenesis toward two transformed cell lines (neuro-2A, PC-12) and one primary neuronal tissue (dorsal root ganglia). The results indicate that the stereochemistry of the ketoside linkage is not critical and that metabolism of exogenous ganglioside by the treated cells is not involved directly in the neuritogenic phenomenon.
先前已表明，多种天然存在的神经节苷脂结构是诱导原代神经元和神经母细胞瘤细胞系神经突生长的有效剂。我们在此报告了使用具有神经氨酸酶抗性β-酮糖苷键的GM3合成差向异构体（表位GM3）进行类似实验的结果。发现该物质可促进两种转化细胞系（Neuro-2A、PC-12）和一种原代神经元组织（背根神经节）的神经突形成。结果表明，酮糖苷键的立体化学并不关键，且处理细胞对外源神经节苷脂的代谢并不直接参与神经突形成现象。
