Instituto de Patobiología Veterinaria (IPVet), Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Hurlingham, Argentina.
Servicios de Enterobacterias, Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán (ANLIS-Malbrán), Buenos Aires, Argentina.
Zoonoses Public Health. 2021 Jun;68(4):309-315. doi: 10.1111/zph.12819. Epub 2021 Feb 16.
In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial.
在过去的 10 年中，肠炎沙门氏菌已在多个国家的家禽中广泛分离。在这种情况下，分子特征分析对于了解菌株是否来自单一或多个来源至关重要。因此，本研究旨在确定 2011 年至 2012 年间从属于阿根廷三家一体化家禽公司的肉鸡养殖场分离的肠炎沙门氏菌菌株的遗传关系和抗菌药物敏感性。通过脉冲场凝胶电泳（PFGE）确定肠炎沙门氏菌分离株的遗传相关性，并用纸片扩散法确定对 21 种抗菌药物的耐药性。分离株被分为四种 PFGE 模式。大多数菌株显示 100%的相似性，属于同一一体化家禽公司。这种 PFGE 模式也在阿根廷几个省份从人类分离的肠炎沙门氏菌菌株中流行，这表明人类和家禽菌株之间存在流行病学关联。所有分离株均被归类为多药耐药（MDR），PFGE 与耐药模式之间未观察到明显关系。由于共同的污染来源，来自同一一体化公司的农场之间可能会循环传播肠炎沙门氏菌菌株。为了保障消费者的家禽产品安全，需要采用整体方法，包括对整个生产链中的沙门氏菌进行监测，并采取控制措施。
