Zhang Jun-Feng, Shang Ke, Park Jong-Yeol, Lee Yea-Jin, Choi Yu-Ri, Kim Sang-Won, Cha Se-Yeoun, Jang Hyung-Kwan, Wei Bai, Kang Min
Department of Veterinary Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Center for Poultry Diseases Control, Jeonbuk National University, Iksan 54596, Korea.
Animals (Basel). 2021 Dec 30;12(1):83. doi: 10.3390/ani12010083.
Antimicrobial resistance and pulsed-field gel electrophoresis (PFGE) genotypes of collected ser. Gallinarum isolates were investigated to examine the epidemiological relationship between field outbreak isolates of ser. Gallinarum. Thirty ser. Gallinarum isolates collected from poultry farms with FT outbreaks from 2013 to 2018 in South Korea were analyzed. All isolates were resistant to at least 3 of the 18 antimicrobials tested and exhibited an MDR phenotype. All isolates showed resistance to streptomycin, sulfisoxazole, and colistin. One isolate was resistant to 9 antimicrobials. The antimicrobial resistance profile, streptomycin-sulfisoxazole-colistin-nalidixic acid-ciprofloxacin-gentamicin (18/30, 60.0%), was the most prevalent. PFGE types were classified into 10 groups with a 100% correlation cutoff in dendrograms for 30 field isolates. The dominant PFGE types were 1 (8/30, 26.7%), 4 (7/30, 23.3%), and 9 (5/30, 16.7%). Interestingly some isolates collected from the same and different companies had the same PFGE type. We reported a high MDR rate in ser. Gallinarum isolates. The present study highlights the occurrence of horizontal spread and cyclic contamination of MDR ser. Gallinarum within the same company. Furthermore, we showed cross-contamination between different companies. The characterization of these isolates would be helpful in the development of prevention and control strategies for MDR ser. Gallinarum infection in South Korea.
对收集的鸡白痢血清型分离株的抗菌药物耐药性和脉冲场凝胶电泳（PFGE）基因型进行了研究，以检验鸡白痢血清型野外暴发分离株之间的流行病学关系。分析了2013年至2018年从韩国发生FT疫情的家禽养殖场收集的30株鸡白痢血清型分离株。所有分离株对所测试的18种抗菌药物中的至少3种耐药，并表现出多重耐药（MDR）表型。所有分离株均对链霉素、磺胺异恶唑和黏菌素耐药。一株分离株对9种抗菌药物耐药。最常见的抗菌药物耐药谱为链霉素-磺胺异恶唑-黏菌素-萘啶酸-环丙沙星-庆大霉素（18/30，60.0%）。在30株野外分离株的树形图中，PFGE类型被分为10组，相关性截止值为100%。主要的PFGE类型为1型（8/30，26.7%）、4型（7/30，23.3%）和9型（5/30，16.7%）。有趣的是，从同一家公司和不同公司收集的一些分离株具有相同的PFGE类型。我们报告了鸡白痢血清型分离株的高多重耐药率。本研究强调了同一公司内多重耐药鸡白痢血清型的水平传播和循环污染的发生。此外，我们还显示了不同公司之间的交叉污染。这些分离株的特征将有助于制定韩国多重耐药鸡白痢血清型感染的预防和控制策略。
