Department of Anatomy, All India Institute of Medical Sciences, Mangalagiri, Andhra Pradesh, India.
Department of Anatomy, Andaman and Nicobar Institute of Medical Sciences, Port Blair, Andaman and Nicobar Islands, India.
J Basic Clin Physiol Pharmacol. 2021 Feb 12;32(5):1007-1012. doi: 10.1515/jbcpp-2020-0264.
Thousands of food additives are being used by the food industries in ready to eat packed food. These food additives act as either preservatives or enhancers of palatability. Monosodium glutamate (MSG), a sodium salt of glutamic acid is a widely used food additive for enhancing taste. It gives the unique taste to the food called "Umami" or "Savory taste", which is different from four primary tastes namely, sweet, sour, salty, and bitter. The current experimental study was investigated by dose-related effects of MSG on adult Wistar rats using histological and histomorphometric techniques.
Twenty-eight adult Wistar rats were divided into four groups i.e.; one control and three experimental groups. Rats were administered orally with different doses of MSG to the experimental groups and distilled water to the control groups consecutively for 45 days. At the end of the study, rats were sacrificed and tissues were collected for the examination.
Histomorphometric data of the nuclei diameter of hepatocytes showed significant variation between control and experimental groups. Less PAS-positive material found in a higher dose of MSG-induced rats in histochemical observation.
One of the challenges of the problem of animal experimental studies is the application of results to human dietary intake of MSG. Based on the dose-dependent findings of the current study; it is evident that the administration of MSG is hepatotoxic in adult Wistar rats.
食品工业在即食包装食品中使用了数千种食品添加剂。这些食品添加剂既可以作为防腐剂，也可以作为提高口感的增强剂。谷氨酸单钠（MSG）是谷氨酸的钠盐，是一种广泛用于增强味道的食品添加剂。它赋予食物独特的味道，称为“鲜味”或“美味”，与甜、酸、咸、苦四种基本味道不同。本实验研究采用组织学和组织形态计量学技术，研究了 MSG 对成年 Wistar 大鼠的剂量相关作用。
将 28 只成年 Wistar 大鼠分为四组，一组为对照组，三组为实验组。实验组大鼠连续口服不同剂量的 MSG，对照组大鼠口服蒸馏水，连续 45 天。研究结束时，处死大鼠并收集组织进行检查。
肝细胞核直径的组织形态计量数据显示，对照组和实验组之间存在显著差异。在组织化学观察中，较高剂量 MSG 诱导的大鼠中发现较少的 PAS 阳性物质。
动物实验研究问题的挑战之一是将研究结果应用于人类对 MSG 的膳食摄入量。基于本研究的剂量依赖性发现，MSG 的给药对成年 Wistar 大鼠具有肝毒性。
