Osmani S A, Pu R T, Morris N R
Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Cell. 1988 Apr 22;53(2):237-44. doi: 10.1016/0092-8674(88)90385-6.
There may be a causal relationship between expression of the G2-specific gene nimA and mitotic regulation in Aspergillus. To test this relationship we have introduced extra inducible copies of nimA into Aspergillus and determined the effect of nimA overproduction on mitotic regulation. The results show that nimA overexpression causes mitotic induction in less than a cell cycle and maintains chromatin in a condensed state. These effects occur even if cells are first blocked in S phase. Sequence analysis shows that the nimA gene encodes a potential protein kinase. These data indicate that there is indeed a causal relationship between expression of nimA and the regulation of mitosis and further implicate protein phosphorylation in mitotic control.
在曲霉中，G2特异性基因nimA的表达与有丝分裂调控之间可能存在因果关系。为了验证这种关系，我们将额外的可诱导nimA拷贝导入曲霉中，并确定nimA过量表达对有丝分裂调控的影响。结果表明，nimA的过表达在不到一个细胞周期内就会引发有丝分裂诱导，并使染色质保持浓缩状态。即使细胞首先被阻滞在S期，这些效应依然会出现。序列分析表明，nimA基因编码一种潜在的蛋白激酶。这些数据表明，nimA的表达与有丝分裂调控之间确实存在因果关系，并且进一步表明蛋白磷酸化参与了有丝分裂控制。
