Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, Viale Michel 11, 15121 Alessandria, Italy.
Dalton Trans. 2021 Mar 7;50(9):3161-3177. doi: 10.1039/d0dt04051g. Epub 2021 Feb 17.
Two Pt(iv) conjugates containing one or two molecules of perillic acid (4-isopropenylcyclohexene-1-carboxylic acid), an active metabolite of limonene, were synthesized both with traditional and microwave-assisted methods and characterized. Their antiproliferative activity was tested on a panel of human tumor cell lines. In particular, cis,cis,trans-[PtCl(NH)(perillato)] exhibited excellent antiproliferative and antimetastatic activity on A-549 lung tumor cells at nanomolar concentrations. A number of in vitro biological tests were performed to decipher some aspects of its mechanism of action, including transwell migration and invasion as well as wound healing assay.
合成了两种含有一个或两个薄荷酸（4-异丙烯基环己烯-1-羧酸）分子的 Pt(iv) 配合物，薄荷酸是柠檬烯的一种活性代谢物，分别采用传统方法和微波辅助方法进行了合成和表征。它们的抗增殖活性在一组人类肿瘤细胞系上进行了测试。特别是顺式、顺式、反式-[PtCl(NH)(perillato)]在纳摩尔浓度下对 A-549 肺肿瘤细胞表现出优异的抗增殖和抗转移活性。进行了多项体外生物学测试来阐明其作用机制的一些方面，包括 Transwell 迁移和侵袭以及伤口愈合测定。
