Key Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing, 100084, P.R. China.
Key Laboratory of Ministry of Education for Traditional Chinese Medicine Resource and Compound Prescription, Hubei University of Chinese Medicine, Wuhan, 430065, P.R. China.
J Sep Sci. 2021 May;44(9):1843-1851. doi: 10.1002/jssc.202001083. Epub 2021 Mar 8.
For a rapid enrichment and separation of minor components from Malus hupehensis, the selection of suitable solvent system is the great challenge for liquid-liquid extraction with a three-phase solvent system and high-speed counter-current chromatography. According to the concept of "like dissolves like," the similarity of the average polarity between solvent system and target compounds was the significant characteristic of liquid-liquid extraction with a three-phase solvent system and high-speed counter-current chromatography separation. The polarity parameter model provides a way to calculate the polarity of unknown compounds. Under the guidance of the polarity, an efficient enrichment and separation approach was established through liquid-liquid extraction and high-speed counter-current chromatography with solvent systems composed of n-hexane-ethyl acetate-acetonitrile-water (5:3:5:7, v/v), n-hexane-ethyl acetate-methanol-water (1:2:1:2, v/v), respectively. Thus, the total content of minor compounds was increased from 2.6% to 17.2%, and two novel compounds (6´´-O-coumaroyl-2´-O-glucopyranosylphloretin and 3´´´-methoxy-6´´-O-feruloy-2´-glucopyranosylphloretin) were obtained. The discovery of the new dihydrochalcones expanded the structural diversity of compounds produced by the genus Malus. The experimental results demonstrated that compound polarity can be described by the polarity parameter model and is an important reference for investigating optimum solvent systems for liquid-liquid extraction with a three-phase solvent system and high-speed counter-current chromatography.
为了从苹果属植物中快速富集和分离微量成分，选择合适的溶剂系统是三相溶剂体系液液萃取和高速逆流色谱分离的巨大挑战。根据“相似相溶”的概念，溶剂系统与目标化合物之间的平均极性相似性是三相溶剂体系液液萃取和高速逆流色谱分离的显著特征。极性参数模型为计算未知化合物的极性提供了一种方法。在极性的指导下，通过分别由正己烷-乙酸乙酯-乙腈-水（5:3:5:7，v/v）和正己烷-乙酸乙酯-甲醇-水（1:2:1:2，v/v）组成的溶剂系统进行液液萃取和高速逆流色谱，建立了一种有效的富集和分离方法。因此，微量化合物的总量从 2.6%增加到 17.2%，并获得了两种新化合物（6´´-O-咖啡酰基-2´-O-吡喃葡萄糖基根皮素和 3´´´-甲氧基-6´´-O-阿魏酰基-2´-吡喃葡萄糖基根皮素）。新二氢查耳酮的发现扩展了苹果属植物产生的化合物的结构多样性。实验结果表明，化合物的极性可以用极性参数模型来描述，这是研究三相溶剂体系液液萃取和高速逆流色谱最佳溶剂系统的重要参考。
