Department of Seafood Processing, Faculty of Fisheries, Recep Tayyip Erdoğan University, 53040, Rize, Turkey.
Department of Freshwater Biology, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey.
Arch Microbiol. 2021 Jul;203(5):2075-2085. doi: 10.1007/s00203-021-02187-9. Epub 2021 Feb 17.
Bioremediation is widely used to remove water pollution as environmentally friendly smart solutions. In this study, Bacillus isolates were investigated in terms of the effectiveness of single and multiple cultures in eliminating aquatic pollution related to aquaculture activities. In the established experimental setups, the environments where Bacillus isolates were inoculated with single and multiple cultures at 1 × 10 CFU/mL were evaluated comparatively with control groups without these isolates, and total aerobic mesophilic bacterial counts were performed in the petri dish by inoculation method. At the end of the 6 days of the experiment, in the environment in which single and multiple cultures of Bacillus isolates were presented with 17-20 ± 0.05 °C temperature and 5.1-8.1 pH 2-4.6 mg/l dissolved oxygen values (O), 2% increase in total phosphorus (TP) value was observed. On the other hand, 4% removal of Ammonia-nitrogen (NH-N), 80% removal of Nitrite-nitrogen (NO-N), and 100% removal of Nitrate-nitrogen (NO-N) were observed. In the changes in heavy metal concentrations, the removal of Ni, Cr, Se, Al, Cd, Mn, Fe, and B was observed from highest to lowest as 57%, 50%, 50%, 43%, 40%, 23%, 5%, and 2%, respectively. It also has been seen that B. thuringiensis isolate was observed to be more effective than B. subtilis in metal removal.
生物修复被广泛应用于去除水污染，作为环保的智能解决方案。在本研究中，研究了芽孢杆菌分离物在单一和多种培养物消除与水产养殖活动有关的水生污染方面的有效性。在建立的实验装置中，比较了在 1×10 CFU/mL 下接种单一和多种芽孢杆菌分离物的环境与没有这些分离物的对照组，通过接种法在培养皿中进行总需氧嗜温细菌计数。在实验的第 6 天结束时，在单一和多种芽孢杆菌分离物培养物存在 17-20±0.05°C 温度和 5.1-8.1 pH 2-4.6mg/l 溶解氧值（O）的环境中，总磷（TP）值增加了 2%。另一方面，观察到氨氮（NH-N）去除 4%、亚硝酸盐氮（NO-N）去除 80%和硝酸盐氮（NO-N）去除 100%。在重金属浓度变化方面，观察到 Ni、Cr、Se、Al、Cd、Mn、Fe 和 B 的去除率从高到低依次为 57%、50%、50%、43%、40%、23%、5%和 2%。还观察到苏云金芽孢杆菌分离物在去除金属方面比枯草芽孢杆菌更有效。
