Usmonkulova Aziza, Malusa Eligio, Kadirova Gulchekhra, Khalilov Ilkhom, Canfora Loredana, Abdulmyanova Liliya
Institute of Microbiology of Academy Sciences of the Republic of Uzbekistan, st. A. Kadyri 7B, 100128 Tashkent, Uzbekistan.
Scientific Research Institute of the Plant Protection and Quarantine, st. Bobur 4., 111215 Tashkent, Uzbekistan.
Microorganisms. 2025 Jun 26;13(7):1485. doi: 10.3390/microorganisms13071485.
In this study, Ni and Cd resistant 18, 11Uz, and Uz_5 strains were isolated from soils contaminated with heavy metals in the Samarkand and Kashkadarya regions (Uzbekistan), and tested to remove Ni and Cd ions from the environment via biosorption. The biosorption capacity of these strains was observed under in vitro conditions. The biosorption process was highly dependent on the growing conditions, with the highest biosorption rate observed after 300 min of incubation at pH 7.0, and 40 °C. The presence of functional groups such as S=O, NH, and COOH in the biosorbing microorganisms was confirmed by IR spectroscopy. The adsorption capacity decreased when the initial metal concentration was increased and was enhanced with higher microbial biomass. 11Uz strain was found to alter the toxic oxidation state of Ni and Cd cations, while 18 and Uz_5 strains reduced the toxicity of Ni cations only by changing their oxidation state. It was confirmed in our studies that the three selected bacterial strains actively participated in the detoxification of Cd through the synthesis of cysteine amino acid.
在本研究中，从乌兹别克斯坦撒马尔罕和卡什卡达里亚地区受重金属污染的土壤中分离出耐镍和镉的18、11Uz和Uz_5菌株，并通过生物吸附测试其从环境中去除镍和镉离子的能力。在体外条件下观察了这些菌株的生物吸附能力。生物吸附过程高度依赖于生长条件，在pH 7.0和40℃孵育300分钟后观察到最高生物吸附率。通过红外光谱证实了生物吸附微生物中存在S=O、NH和COOH等官能团。当初始金属浓度增加时，吸附容量降低，而较高的微生物生物量则增强了吸附容量。发现11Uz菌株改变了镍和镉阳离子的有毒氧化态，而18和Uz_5菌株仅通过改变其氧化态降低了镍阳离子的毒性。我们的研究证实，所选的三种细菌菌株通过合成半胱氨酸积极参与镉的解毒过程。
