Provincial Laboratory for Public Health (ProvLab), Alberta Precision Laboratories, Edmonton, Alberta, Canada.
Department of Medical Microbiology and Immunology, University of Alberta, 2B3.13 Walter Mackenzie Health Sciences Centre, University of Alberta Hospital, 8440 112 Street, Edmonton, AB, T6G 2J2, Canada.
Med Microbiol Immunol. 2021 Feb;210(1):73-80. doi: 10.1007/s00430-021-00699-1. Epub 2021 Feb 17.
The focus on urogenital mycoplasmas as the possible etiologic agents of urogenital infections and syndromes, has increased in the last decade. Of these, Mycoplasma genitalium is proven to be pathogenic and sexually transmitted. We compared five commercially available assays for the detection of these organisms in urogenital mycoplasma culture specimen remnants. Stored specimen remnants were tested on Aptima Mycoplasma genitalium, Allplex™ STI Essential and CGMT, ResitancePlusMG and Allplex™ MG & AziR Assays. All positive M. genitalium specimens and culture negative, nucleic acid positive Ureaplasmas were sent to the National Microbiology Laboratory for confirmation. The Aptima Mycoplasma genitalium assay detected 7 M. genitalium infections, the Allplex™ STI-EA and the Allplex™ CGMT detected 6 M. genitalium positives, and the Allplex™MG and AziR and SpeeDx ResistancePlus MG detected 5 M. genitalium positives, four with macrolide resistant genes. The Allplex™ STI Essential assay was 100% sensitive and specific for Mycoplasma hominis and Ureaplasma targets. As seen in other studies, the Aptima Mycoplasma genitalium assay was 100% sensitive and specific for the detection of M. genitalium. The multiplex assays had lower sensitivities for M. genitalium detection (Allplex™ STI Essential and CGMT sensitivity of 85.71%; Allplex™ MG & AziR and SpeeDx ResistancePlus MG sensitivity of 71.43%) with high specificities of 100%. Assays tested have high sensitivities and specificities for the detection of urogenital mycoplasmas especially M. genitalium macrolide resistance markers. All labs wanting to perform onsite detection of these organisms will find an assay to easily fit into their workflow.
在过去的十年中，人们越来越关注泌尿生殖支原体作为泌尿生殖系统感染和综合征的可能病原体。在这些支原体中，已证实生殖支原体具有致病性和性传播性。我们比较了五种市售的检测试剂盒，用于检测泌尿生殖支原体培养标本残留物中的这些微生物。将储存的标本残留物用于 Aptima 生殖道支原体、Allplex™ STI 基本型和 CGMT、ResitancePlusMG 和 Allplex™ MG & AziR 检测。所有阳性的生殖道支原体标本和培养阴性、核酸阳性的脲原体均被送往国家微生物学实验室进行确认。Aptima 生殖道支原体检测法检测到 7 例生殖道支原体感染，Allplex™ STI-EA 和 Allplex™ CGMT 检测到 6 例生殖道支原体阳性，Allplex™ MG & AziR 和 SpeeDx ResistancePlus MG 检测到 5 例生殖道支原体阳性，其中 4 例具有大环内酯类耐药基因。Allplex™ STI 基本型检测法对人型支原体和脲原体靶标具有 100%的敏感性和特异性。与其他研究一样，Aptima 生殖道支原体检测法对生殖道支原体的检测具有 100%的敏感性和特异性。多重检测法对生殖道支原体的检测敏感性较低（Allplex™ STI 基本型和 CGMT 的敏感性为 85.71%；Allplex™ MG & AziR 和 SpeeDx ResistancePlus MG 的敏感性为 71.43%），特异性均为 100%。这些检测方法对泌尿生殖支原体，尤其是生殖支原体大环内酯类耐药标志物的检测具有较高的敏感性和特异性。所有希望进行这些微生物现场检测的实验室都将找到一种易于融入其工作流程的检测方法。
