评价两种快速表型检测方法——Alifax 快速 AST 黏菌素检测法和 Rapid Polymyxin NP 检测法——用于检测肠杆菌科对黏菌素的耐药性。
Evaluation of two rapid phenotypical tests-Alifax rapid AST colistin test and Rapid Polymyxin NP test-for detection of colistin resistance in Enterobacterales.
机构信息
Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
Diagnostic & Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.
出版信息
Eur J Clin Microbiol Infect Dis. 2021 Aug;40(8):1749-1753. doi: 10.1007/s10096-021-04182-w. Epub 2021 Feb 17.
Abstract
Our study evaluates the performance of two rapid phenotypical tests to detect colistin resistance in Enterobacterales: Alifax rapid AST colistin test using the HB&L system and Rapid Polymyxin NP test prepared in-house. A collection of well-characterized 53 colistin-susceptible and 66 colistin-resistantEnterobacterales isolates was used. The results obtained using both rapid tests were compared to the reference broth microdilution. Overall categorical agreement was 81.5% for Alifax test and 98.3% for Rapid Polymyxin NP test. Based on our results, the Rapid Polymyxin NP test is superior to the Alifax test that performed inadequate for Enterobacter spp.
摘要
我们的研究评估了两种快速表型检测方法在检测肠杆菌科中多粘菌素耐药性的性能
使用 HB&L 系统的 Alifax 快速 AST 多粘菌素检测和内部制备的 Rapid Polymyxin NP 检测。使用了一组经过充分特征描述的 53 株多粘菌素敏感和 66 株多粘菌素耐药肠杆菌科分离株。这两种快速检测方法的结果与参考肉汤微量稀释法进行了比较。Alifax 试验的总体分类一致性为 81.5%，Rapid Polymyxin NP 试验为 98.3%。根据我们的结果，Rapid Polymyxin NP 试验优于 Alifax 试验，因为后者对肠杆菌属表现出不足。
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