Christian Medical Research Center, Presbyterian Medical Center, Jeonju, Korea.
Division of Nephrology, Department of Internal Medicine, Presbyterian Medical Center, Jeonju, Korea.
Genet Test Mol Biomarkers. 2021 Feb;25(2):140-144. doi: 10.1089/gtmb.2020.0238.
Urinary microRNA (miRNA)-21 is a biomarker for acute kidney injury (AKI). We conducted this study to determine if a urinary exosomal analysis for this biomarker could serve as a novel diagnostic approach for detecting kidney disease. We investigated the clinical significance of urinary exosomal miRNA-21 levels for AKI in scrub typhus patients. We collected 138 urine samples from scrub typhus patients at the time of admission. Urinary exosomal miRNA-21 was assessed in 25 age- and sex-matched scrub typhus patients with and without AKI. The total leukocyte count was higher in AKI patients than in non-AKI patients (10.40 × 10/mL vs. 6.40 × 10/mL, < 0.01). Urinary exosomal miRNA-21 levels were higher in the AKI group than in the non-AKI group (20.1 ± 1.2 vs. 17.8 ± 1.8 ΔCt value of miRNA-21, < 0.01). Additionally, the miRNA-21 levels correlated directly with the total leukocyte counts and inversely with the estimated glomerular filtration rate. A receiver operating characteristic curve analysis demonstrated good discriminative power for the diagnosis of scrub typhus-associated AKI, with an area under the curve value of 0.907. Urinary exosomal miRNA-21 could be a surrogate marker for scrub typhus-associated AKI diagnosis.
尿 microRNA（miRNA）-21 是急性肾损伤（AKI）的生物标志物。我们进行这项研究，以确定这种生物标志物的尿外泌体分析是否可以作为一种新的诊断方法，用于检测肾脏疾病。我们研究了恙虫病患者尿外泌体 miRNA-21 水平对 AKI 的临床意义。我们在入院时收集了 138 例恙虫病患者的尿液样本。在 25 例年龄和性别匹配的恙虫病患者中，评估了有无 AKI 的患者的尿外泌体 miRNA-21。AKI 患者的白细胞总数高于非 AKI 患者（10.40×10/mL 比 6.40×10/mL， < 0.01）。AKI 组的尿外泌体 miRNA-21 水平高于非 AKI 组（20.1±1.2 比 17.8±1.8 ΔCt 值的 miRNA-21， < 0.01）。此外，miRNA-21 水平与白细胞总数呈正相关，与估算肾小球滤过率呈负相关。受试者工作特征曲线分析表明，对于诊断恙虫病相关 AKI，具有良好的区分能力，曲线下面积值为 0.907。尿外泌体 miRNA-21 可能是恙虫病相关 AKI 诊断的替代标志物。
