Beijing Advanced Innovation Center for Food Nutrition and Human Health/Beijing Key Lab of Plant Resource Research and Development, Institute of Cosmetic Regulatory Science, Beijing Technology and Business University, Beijing, 100048, China.
Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS)/National Key Facility for Crop Gene Resources and Genetic Improvement, Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing, 100081, China.
Sci Rep. 2021 Feb 17;11(1):4024. doi: 10.1038/s41598-021-83440-5.
Plant-specific WRKY transcription factors play important roles in regulating the expression of defense-responsive genes against pathogen attack. A multiple stress-responsive WRKY gene, ZmWRKY65, was identified in maize by screening salicylic acid (SA)-induced de novo transcriptomic sequences. The ZmWRKY65 protein was localized in the nucleus of mesophyll protoplasts. The analysis of the ZmWRKY65 promoter sequence indicated that it contains several stress-related transcriptional regulatory elements. Many environmental factors affecting the transcription of ZmWRKY65 gene, such as drought, salinity, high temperature and low temperature stress. Moreover, the transcription of ZmWRKY65 gene was also affected by the induction of defense related plant hormones such as SA and exogenous ABA. The results of seed germination and stomatal aperture assays indicated that transgenic Arabidopsis plants exhibit enhanced sensitivity to ABA and high concentrations of SA. Overexpression of ZmWRKY65 improved tolerance to both pathogen attack and abiotic stress in transgenic Arabidopsis plants and activated several stress-related genes such as RD29A, ERD10, and STZ as well as pathogenesis-related (PR) genes such as PR1, PR2 and PR5; these genes are involved in resistance to abiotic and biotic stresses in Arabidopsis. Together, this evidence implies that the ZmWRKY65 gene is involved in multiple stress signal transduction pathways.
植物特异性 WRKY 转录因子在调节防御相关基因的表达方面发挥着重要作用，可响应病原体攻击。本研究通过筛选水杨酸（SA）诱导的从头转录组序列，从玉米中鉴定出一个多胁迫响应 WRKY 基因 ZmWRKY65。ZmWRKY65 蛋白定位于叶肉原生质体的细胞核中。ZmWRKY65 启动子序列分析表明，它含有几个与胁迫相关的转录调控元件。许多环境因素影响 ZmWRKY65 基因的转录，如干旱、盐胁迫、高温和低温胁迫。此外，防御相关植物激素如 SA 和外源 ABA 的诱导也会影响 ZmWRKY65 基因的转录。种子萌发和气孔开度测定的结果表明，转 ZmWRKY65 基因拟南芥对 ABA 和高浓度 SA 表现出更高的敏感性。过表达 ZmWRKY65 可提高转基因拟南芥对病原体攻击和非生物胁迫的耐受性，并激活一些胁迫相关基因，如 RD29A、ERD10 和 STZ，以及病程相关（PR）基因，如 PR1、PR2 和 PR5；这些基因参与拟南芥的非生物和生物胁迫抗性。综上所述，这些证据表明 ZmWRKY65 基因参与了多种胁迫信号转导途径。
