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1,25-二羟基维生素D对鸡间充质干细胞成骨分化和矿化的作用

Role of 1,25-Dihydroxyvitamin D on Osteogenic Differentiation and Mineralization of Chicken Mesenchymal Stem Cells.

作者信息

Chen Chongxiao, Adhikari Roshan, White Dima Lynn, Kim Woo Kyun

机构信息

Department of Poultry Science, University of Georgia, Athens, GA, United States.

出版信息

Front Physiol. 2021 Feb 1;12:479596. doi: 10.3389/fphys.2021.479596. eCollection 2021.

DOI:10.3389/fphys.2021.479596
PMID:33597893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7882605/
Abstract

1,25-dihydroxyvitamin D3 (1,25OHD) has been suggested to play an important role in osteogenic differentiation and mineralization. However, limited data have been reported in avian species. In the present study, the direct role of 1,25OHD on osteogenic differentiation and mineralization in chicken mesenchymal stem cells (cMSCs) derived from day-old broiler bones was investigated. cMSCs were treated with control media (C), osteogenesis media (OM), OM with 1, 5, 10, and 50 nM 1,25OHD, respectively. The messenger RNA (mRNA) samples were obtained at 24 and 48 h and 3 and 7 days to examine mRNA expression of key osteogenic genes [runt related transcription factor 2 (RUNX2), bone morphogenetic protein 2 (BMP2), collagen type I alpha 2 chain (COL1A2), bone gamma-carboxyglutamate protein (BGLAP), secreted phosphoprotein 1 (SPP1), and alkaline phosphatase (ALP)]. Cells were stained at 7, 14, and 21 days using Von Kossa (mineralization), Alizarin Red (AR; mineralization), and Alkaline Phosphatase (early marker) staining methods. From the mRNA expression results, we found a time-dependent manner of 1,25OHD on osteoblast differentiation and mineralization. In general, it showed an inhibitory effect on differentiation and mineralization during the early stage (24 and 48 h), and a stimulatory effect during the late cell stage (3 and 7 days). The staining showed 1,25OHD had an inhibitory effect on ALP enzyme activities and mineralization in a dosage-dependent manner up to 14 days. However, at 21 days, there was no difference between the treatments. This study provides a novel understanding of the effects of 1,25OHD on osteogenic differentiation and mineralization of cMSCs depending on cell stage and maturity.

摘要

1,25-二羟基维生素D3（1,25OHD）被认为在成骨分化和矿化过程中发挥重要作用。然而，关于禽类的相关数据报道有限。在本研究中，我们探究了1,25OHD对源自一日龄肉鸡骨骼的鸡间充质干细胞（cMSCs）成骨分化和矿化的直接作用。cMSCs分别用对照培养基（C）、成骨培养基（OM）、添加1、5、10和50 nM 1,25OHD的OM处理。在24小时和48小时以及3天和7天时获取信使核糖核酸（mRNA）样本，以检测关键成骨基因[ runt相关转录因子2（RUNX2）、骨形态发生蛋白2（BMP2）、I型胶原α2链（COL1A2）、骨γ-羧基谷氨酸蛋白（BGLAP）、分泌性磷蛋白1（SPP1）和碱性磷酸酶（ALP）]的mRNA表达。在第7、14和21天使用冯科萨染色法（矿化）、茜素红染色法（AR；矿化）和碱性磷酸酶染色法（早期标志物）对细胞进行染色。从mRNA表达结果来看，我们发现1,25OHD对成骨细胞分化和矿化具有时间依赖性作用。总体而言，在早期阶段（24小时和48小时）它对分化和矿化表现出抑制作用，而在细胞后期阶段（3天和7天）则表现出刺激作用。染色结果显示，直至第14天，1,25OHD对ALP酶活性和矿化具有剂量依赖性抑制作用。然而，在第21天，各处理组之间没有差异。本研究为1,25OHD根据细胞阶段和成熟度对cMSCs成骨分化和矿化的影响提供了新的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/38b0cdbd54a4/fphys-12-479596-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/7a407af4ff98/fphys-12-479596-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/3857fb8d5bca/fphys-12-479596-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/72af4aa47644/fphys-12-479596-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/841cee0ef77e/fphys-12-479596-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/5fcab46efd64/fphys-12-479596-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/d48543d29880/fphys-12-479596-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/38b0cdbd54a4/fphys-12-479596-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/7a407af4ff98/fphys-12-479596-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/3857fb8d5bca/fphys-12-479596-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/72af4aa47644/fphys-12-479596-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/841cee0ef77e/fphys-12-479596-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/5fcab46efd64/fphys-12-479596-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/d48543d29880/fphys-12-479596-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/552a/7882605/38b0cdbd54a4/fphys-12-479596-g007.jpg

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引用本文的文献

[1]
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J Dev Biol. 2025-6-1

[2]
Phytase, 25-hydroxyvitamin D and cocci vaccination to broilers fed a calcium and phosphorus-reduced diet under Eimeria spp. challenge: effects on growth performance and intestinal health.

Poult Sci. 2024-12

[3]
Altered Osteogenic Differentiation in Mesenchymal Stem Cells Isolated from Compact Bone of Chicken Treated with Varying Doses of Lipopolysaccharides.

Biomolecules. 2023-11-7

[4]
A Review on Pathophysiology, and Molecular Mechanisms of Bacterial Chondronecrosis and Osteomyelitis in Commercial Broilers.

Biomolecules. 2023-6-23

[5]
Effect of the combination of 25-hydroxyvitamin D3 and higher level of calcium and phosphorus in the diets on bone 3D structural development in pullets.

Front Physiol. 2023-4-24

[6]
Effects of rearing systems on the eggshell quality, bone parameters and expression of genes related to bone remodeling in aged laying hens.

Front Physiol. 2022-8-31

本文引用的文献

[1]
Isolation and Differentiation of Mesenchymal Stem Cells From Broiler Chicken Compact Bones.

Front Physiol. 2019-1-22

[2]
Vitamin D: Classic and Novel Actions.

Ann Nutr Metab. 2018-1-18

[3]
1α,25-Dihydroxyvitamin D promotes osteogenesis by promoting Wnt signaling pathway.

J Steroid Biochem Mol Biol. 2017-11

[4]
Vitamin D Activity and Metabolism in Bone.

Curr Osteoporos Rep. 2017-10

[5]
Synergistic effect of 1α,25-dihydroxyvitamin D and 17β-estradiol on osteoblast differentiation of pediatric MSCs.

J Steroid Biochem Mol Biol. 2017-7-29

[6]
Adrenocorticotropic hormone and 1,25-dihydroxyvitamin D enhance human osteogenesis in vitro by synergistically accelerating the expression of bone-specific genes.

Lab Invest. 2017-9

[7]
Downregulation of Runx2 by 1,25-Dihydroxyvitamin D₃ Induces the Transdifferentiation of Osteoblasts to Adipocytes.

Int J Mol Sci. 2016-5-19

[8]
Super pharmacological levels of calcitriol (1,25-(OH)2D3) inhibits mineral deposition and decreases cell proliferation in a strain dependent manner in chicken mesenchymal stem cells undergoing osteogenic differentiation in vitro.

Poult Sci. 2015-11

[9]
Runx2: Structure, function, and phosphorylation in osteoblast differentiation.

Int J Biol Macromol. 2015-4-13

[10]
Vitamin D endocrine system and osteoblasts.

Bonekey Rep. 2014-2-5

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