文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

不同剂量脂多糖处理鸡密质骨间充质干细胞成骨分化的改变。

Altered Osteogenic Differentiation in Mesenchymal Stem Cells Isolated from Compact Bone of Chicken Treated with Varying Doses of Lipopolysaccharides.

机构信息

Department of Poultry Science, University of Georgia, Athens, GA 30605, USA.

出版信息

Biomolecules. 2023 Nov 7;13(11):1626. doi: 10.3390/biom13111626.

DOI:10.3390/biom13111626
PMID:38002308
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10669906/
Abstract

Persistent inflammation biologically alters signaling molecules and ultimately affects osteogenic differentiation, including in modern-day broilers with unique physiology. Lipopolysaccharides (LPS) are Gram-negative bacterial components that activate cells via transmembrane receptor activation and other molecules. Previous studies have shown several pathways associated with osteogenic inductive ability, but the pathway has yet to be deciphered, and data related to its dose-dependent effect are limited. Primary mesenchymal stem cells (MSCs) were isolated from the bones of day-old broiler chickens, and the current study focused on the dose-dependent variation (3.125 micrograms/mL to 50 micrograms/mL) in osteogenic differentiation and the associated biomarkers in primary MSCs. The doses in this study were determined using a cell viability (MTT) assay. The study revealed that osteogenic differentiation varied with dose, and the cells exposed to higher doses of LPS were viable but lacked differentiating ability. However, this effect became transient with lower doses, and this phenotypic character was observed with differential staining methods like Alizarin Red, Von Kossa, and alkaline phosphatase. The data from this study revealed that LPS at varying doses had a varying effect on osteogenic differentiation via several pathways acting simultaneously during bone development.

摘要

持续的炎症会在生物学上改变信号分子，最终影响成骨分化，包括现代肉鸡这种具有独特生理学特性的动物。脂多糖（LPS）是革兰氏阴性细菌的组成部分，通过跨膜受体激活和其他分子激活细胞。先前的研究表明了与成骨诱导能力相关的几种途径，但该途径尚未被破解，并且关于其剂量依赖性效应的数据有限。原代间充质干细胞（MSCs）是从一日龄肉鸡的骨骼中分离出来的，本研究主要关注成骨分化的剂量依赖性变化（3.125 微克/毫升至 50 微克/毫升）以及原代 MSCs 中的相关生物标志物。本研究中的剂量是通过细胞活力（MTT）测定确定的。研究表明，成骨分化随剂量而变化，暴露于较高 LPS 剂量的细胞虽然存活但缺乏分化能力。然而，这种效应在较低剂量下是短暂的，并且这种表型特征可以通过茜素红、Von Kossa 和碱性磷酸酶等差异染色方法观察到。这项研究的数据表明，LPS 在不同剂量下通过在骨骼发育过程中同时作用的几种途径对成骨分化产生不同的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/80daf945060a/biomolecules-13-01626-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/83c748a4e454/biomolecules-13-01626-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/82cedacf8c44/biomolecules-13-01626-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/37d709728b9e/biomolecules-13-01626-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/755d83fab57a/biomolecules-13-01626-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/c9c25a433114/biomolecules-13-01626-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/08ca231d047e/biomolecules-13-01626-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/c7b64c8eea86/biomolecules-13-01626-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/a0d0964f675e/biomolecules-13-01626-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/cdb21dbe50f1/biomolecules-13-01626-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/80daf945060a/biomolecules-13-01626-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/83c748a4e454/biomolecules-13-01626-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/82cedacf8c44/biomolecules-13-01626-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/37d709728b9e/biomolecules-13-01626-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/755d83fab57a/biomolecules-13-01626-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/c9c25a433114/biomolecules-13-01626-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/08ca231d047e/biomolecules-13-01626-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/c7b64c8eea86/biomolecules-13-01626-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/a0d0964f675e/biomolecules-13-01626-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/cdb21dbe50f1/biomolecules-13-01626-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bee/10669906/80daf945060a/biomolecules-13-01626-g010.jpg

相似文献

[1]
Altered Osteogenic Differentiation in Mesenchymal Stem Cells Isolated from Compact Bone of Chicken Treated with Varying Doses of Lipopolysaccharides.

Biomolecules. 2023-11-7

[2]
Effects of l-Tryptophan and 1,25-Dihydroxycholecalciferol on Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells Isolated from the Compact Bones of Broilers and Layers.

J Agric Food Chem. 2022-8-31

[3]
Effect of 20(S)-Hydroxycholesterol on Multilineage Differentiation of Mesenchymal Stem Cells Isolated from Compact Bones in Chicken.

Genes (Basel). 2020-11-17

[4]
Galangin inhibits lipopolysaccharide-induced inflammation and stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via regulation of AKT/mTOR signaling.

Allergol Immunopathol (Madr). 2023-1-1

[5]
Inhibition of Wnt11 impairs the osteogenesis and aggravates the inflammatory response of human mesenchymal stem cells under LPS-induced inflammatory condition.

Biochem Biophys Res Commun. 2023-6-18

[6]
[Mechanism of ring finger protein 11 regulating Akt signaling pathway to promote osteogenic differentiation of bone marrow mesenchymal stem cells].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2022-1-15

[7]
Lipopolysaccharide from Escherichia coli stimulates osteogenic differentiation of human periodontal ligament stem cells through Wnt/β-catenin-induced TAZ elevation.

Mol Oral Microbiol. 2018-12-11

[8]
Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway.

Stem Cell Res Ther. 2014-5-27

[9]
Inhibitory effect of alcohol on osteogenic differentiation in human bone marrow-derived mesenchymal stem cells.

Alcohol Clin Exp Res. 2004-3

[10]
Osteogenic differentiation of rat mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells: melatonin as a differentiation factor.

Iran Biomed J. 2008-7

引用本文的文献

[1]
Effect of lipopolysaccharides and mixed Eimeria spp. challenge on performance and bone development in broilers.

Poult Sci. 2025-6-27

[2]
Necrotic enteritis affects bone growth and bone microstructure in non-selected conventional and modern meat-type chicken strains.

Poult Sci. 2025-5-26

本文引用的文献

[1]
The Role and Mechanism of MicroRNA 21 in Osteogenesis: An Update.

Int J Mol Sci. 2023-7-11

[2]
A Review on Pathophysiology, and Molecular Mechanisms of Bacterial Chondronecrosis and Osteomyelitis in Commercial Broilers.

Biomolecules. 2023-6-23

[3]
Effect of Hydrogen Oxide-Induced Oxidative Stress on Bone Formation in the Early Embryonic Development Stage of Chicken.

Biomolecules. 2023-1-12

[4]
Effects of mixed Eimeria challenge on performance, body composition, intestinal health, and expression of nutrient transporter genes of Hy-Line W-36 pullets (0-6 wks of age).

Poult Sci. 2022-11

[5]
The Role of Lipopolysaccharide-Induced Cell Signalling in Chronic Inflammation.

Chronic Stress (Thousand Oaks). 2022-2-8

[6]
Study of the effects of Escherichia coli lipopolysaccharide on innate immunity: The expression profile of TLR4 and CD14 genes in rat liver.

Open Vet J. 2021

[7]
Role of 1,25-Dihydroxyvitamin D on Osteogenic Differentiation and Mineralization of Chicken Mesenchymal Stem Cells.

Front Physiol. 2021-2-1

[8]
Biomimetic osteogenic peptide with mussel adhesion and osteoimmunomodulatory functions to ameliorate interfacial osseointegration under chronic inflammation.

Biomaterials. 2020-10

[9]
Double-Stranded RNA Is a Novel Molecular Target in Osteomyelitis Pathogenesis: A Translational Avian Model for Human Bacterial Chondronecrosis with Osteomyelitis.

Am J Pathol. 2019-8-2

[10]
Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway.

Biomed Pharmacother. 2019-3-2

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索