Gottsch J D, Hairston R J, Chen C H, Graham C R, Stark W J
Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Curr Eye Res. 1988 Mar;7(3):253-6. doi: 10.3109/02713688809047030.
This study confirms the feasibility of monitoring corneal metabolism of an amino acid using high resolution NMR spectroscopy. Alanine metabolism to lactate was detected in intact, freshly isolated rabbit corneas. Progressively decreasing alanine resonances were noted after 4-5 hours of incubation while increasing amounts of lactate were detected. No similar signal was noted in de-epithelialized tissue. This study demonstrates the ability of corneal epithelium to utilize alanine as a metabolic substrate. This technique may be useful to determine the essential amino acids and their optimal concentrations necessary to maintain corneal viability during storage.
本研究证实了使用高分辨率核磁共振光谱监测氨基酸角膜代谢的可行性。在完整、新鲜分离的兔角膜中检测到丙氨酸向乳酸的代谢。孵育4 - 5小时后，丙氨酸共振逐渐降低，同时检测到乳酸量增加。在去上皮组织中未观察到类似信号。本研究证明了角膜上皮利用丙氨酸作为代谢底物的能力。该技术可能有助于确定维持角膜储存期间活力所需的必需氨基酸及其最佳浓度。
