Midelfart A, Dybdahl A, Gribbestad I S
Department of Ophthalmology, Faculty of Medicine, Norwegian Institute of Technology, University of Trondheim, Norway.
Ophthalmic Res. 1996;28(5):319-29. doi: 10.1159/000267921.
Perchloric acid extracts of the rabbit cornea were analyzed by high-resolution 1H NMR spectroscopy, including the two-dimensional shift-correlated (COSY) technique. Numerous metabolites including leucine/isoleucine, 3-hydroxybutyrate, valine, lactate, alanine, lysine, acetate, glutamate, creatine/phosphocreatine, taurine, glycine, inositol, glucose, tyrosine, phenylalanine, histidine, adenosine and formate have been simultaneously detected and identified in the NMR spectra. Concentrations of some of these were also determined. Of particular importance are the detection and identification of hypotaurine in the corneal tissue. Hypotaurine is a precursor of the synthesis of taurine and may act as an antioxidant. Moreover, the presence of choline, a precursor for acetylcholine, was established. This novel metabolite profile of the cornea obtained by high-resolution 1H NMR spectroscopy may be of value in the study of pathologic and toxic disorders.
采用高分辨率¹H核磁共振波谱法，包括二维位移相关（COSY）技术，对兔角膜的高氯酸提取物进行了分析。在核磁共振谱中同时检测并鉴定出了多种代谢物，包括亮氨酸/异亮氨酸、3-羟基丁酸、缬氨酸、乳酸、丙氨酸、赖氨酸、乙酸盐、谷氨酸、肌酸/磷酸肌酸、牛磺酸、甘氨酸、肌醇、葡萄糖、酪氨酸、苯丙氨酸、组氨酸、腺苷和甲酸。还测定了其中一些代谢物的浓度。角膜组织中次牛磺酸的检测和鉴定尤为重要。次牛磺酸是牛磺酸合成的前体，可能具有抗氧化作用。此外，还确定了乙酰胆碱前体胆碱的存在。通过高分辨率¹H核磁共振波谱法获得的这种角膜新代谢物谱，可能在病理和毒性疾病的研究中具有价值。
