De Liguoro M, Anfossi P, Angeletti R, Montesissa C
Istituto di Patologia e Igiene Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Padova, AGRIPOLIS, Legnaro, Italy.
Analyst. 1998 Jun;123(6):1279-82. doi: 10.1039/a708004b.
In accordance with the maximum residue limit of 100 micrograms kg-1 established by EU legislation, a simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for the measurement of tylosin residues in pig tissues (fat, kidney, liver and muscle). Tylosin, a macrolide antibiotic, is extracted with water-methanol and cleaned-up by solid-phase extraction (SPE) on cation-exchange cartridges using methanol elution. Tylosin was determined by reversed-phase HPLC with UV detection at 280 nm and the mean recovery from pig tissues fortified in the range 50-200 micrograms kg-1 was 70-85%, with intra- and inter-day RSDs in the ranges 3.4-9.1 and 3.9-10.1% respectively.
根据欧盟法规规定的100微克/千克的最大残留限量，开发了一种简单灵敏的高效液相色谱（HPLC）方法，用于测定猪组织（脂肪、肾脏、肝脏和肌肉）中的泰乐菌素残留量。泰乐菌素是一种大环内酯类抗生素，用水 - 甲醇提取，并用阳离子交换柱进行固相萃取（SPE），以甲醇洗脱进行净化。通过反相HPLC在280nm处进行紫外检测来测定泰乐菌素，在50 - 200微克/千克范围内加标的猪组织中的平均回收率为70 - 85%，日内和日间相对标准偏差（RSD）分别在3.4 - 9.1%和3.9 - 10.1%范围内。
