College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Engineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Huazhong Agricultural University, Wuhan, China.
Anim Genet. 2021 Jun;52(3):311-320. doi: 10.1111/age.13046. Epub 2021 Feb 18.
Chinese perch, Siniperca chuatsi (Basilewsky), is one of the most commercially important cultured fishes in China. In the present study, a high-density genetic linkage map of Chinese perch was constructed by genotyping-by-sequencing technique with an F1 mapping panel containing 190 progenies. A total of 2328 SNPs were assigned to 24 linkage groups (LGs), agreeing with the chromosome haploid number in this species (n = 24). The sex-averaged map covered 97.9% of the Chinese perch genome, with the length of 1694.3 cM and a marker density of 0.7 cM/locus. The number of markers per LG ranged from 57 to 222, with a mean of 97. The length of LGs varied from 43.2 to 108.2 cM, with a mean size of 70.6 cM. The recombination rate of females was 1.5:1, which was higher than that of males. To better understand the distribution pattern of segregation distortion between the two sexes of Chinese perch, the skewed markers were retained and used to reconstruct the sex-specific maps. The 16 segregation distortion regions were identified on 10 LGs of the female map, while 12 segregation distortion regions on eight LGs of the male map. Among these LGs, six LGs matched between the sex-specific maps. This high-density linkage map could provide a solid basis for identifying QTL associated with economically important traits, and for implementing marker-assisted selection breeding of Chinese perch.
中国鲈鱼（Siniperca chuatsi）是中国最重要的养殖鱼类之一。本研究利用基因分型测序技术，构建了一个高密度的中国鲈鱼遗传连锁图谱，其 F1 作图群体由 190 个后代组成。共鉴定到 2328 个 SNP 标记，被分配到 24 个连锁群（LG）上，与该物种的染色体单倍体数目（n = 24）一致。性连锁图谱覆盖了中国鲈鱼基因组的 97.9%，图谱总长度为 1694.3cM，标记间平均距离为 0.7cM/标记。每条 LG 上的标记数从 57 到 222 不等，平均值为 97。LG 长度范围从 43.2 到 108.2cM，平均值为 70.6cM。雌性的重组率为 1.5:1，高于雄性。为了更好地理解中国鲈鱼两性之间分离失真的分布模式，保留了偏分离的标记并用于构建性别特异性图谱。在雌性图谱的 10 个 LG 上鉴定到 16 个分离失真区域，而在雄性图谱的 8 个 LG 上鉴定到 12 个分离失真区域。在这些 LG 中，有 6 个 LG 在性别特异性图谱之间匹配。该高密度连锁图谱可为鉴定与经济重要性状相关的 QTL 提供坚实的基础，为中国鲈鱼的标记辅助选择育种提供支持。
