Department of Gastroenterology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning, China.
Department of Gastroenterology, 967 Hospital of the Joint Logistics Support Force of PLA, Dalian, Liaoning, China.
J Clin Apher. 2021 Jun;36(3):454-464. doi: 10.1002/jca.21887. Epub 2021 Feb 18.
Ulcerative colitis (UC) is a refractory disease with unclear etiology. Studies have shown that UC is closely associated with gut microbiota dysbiosis. Adsorptive granulomonocytapheresis (GMA) using an Adacolumn has been found to treat UC effectively, but its underlying mechanism of treatment has not been fully elucidated. In this study, we aimed to investigate the influence of GMA on the gut microbiota in patients with active UC.
We conducted a single-center prospective analysis of patients with active UC who received GMA therapy and ultimately achieved clinical remission. Stool samples of healthy controls and patients before and after 5 or 10 sessions of GMA therapy were collected. Subsequently, high-throughput sequencing of the 16S rRNA V3 and V4 gene region of the stool was conducted and clustering of operational taxonomic units and species annotation were performed.
Gut microbial profiles in patients with UC were characterized by low bacterial diversity. After 5 or 10 sessions of GMA therapy, the gut microbiota diversity in patients with UC increased and was similar to that of healthy controls. UC was further characterized by increased abundances of Proteobacteria and Bacteroides, as well as decreased abundances of Faecalibacterium, Roseburia, Firmicutes, and Dialister; however, after GMA therapy, the abundance of Bacteroides decreased, whereas those of Faecalibacterium, Roseburia, and Firmicutes increased.
Active UC is associated with gut microbiota dysbiosis. GMA therapy exerts a strong regulatory effect on the gut microbiota in patients with UC.
溃疡性结肠炎（UC）是一种病因不明的难治性疾病。研究表明，UC 与肠道微生物失调密切相关。使用 Adacolumn 进行吸附性粒单核细胞清除术（GMA）已被发现可有效治疗 UC，但其治疗的潜在机制尚未完全阐明。在本研究中，我们旨在研究 GMA 对活动期 UC 患者肠道微生物群的影响。
我们对接受 GMA 治疗并最终达到临床缓解的活动期 UC 患者进行了单中心前瞻性分析。收集健康对照者和 GMA 治疗前 5 或 10 次治疗后患者的粪便样本。随后，对粪便的 16S rRNA V3 和 V4 基因区进行高通量测序，并对操作分类单元进行聚类和物种注释。
UC 患者的肠道微生物谱特征为细菌多样性低。经过 5 或 10 次 GMA 治疗后，UC 患者的肠道微生物多样性增加，与健康对照组相似。UC 还表现为 Proteobacteria 和 Bacteroides 的丰度增加，而 Faecalibacterium、Roseburia、Firmicutes 和 Dialister 的丰度降低；然而，经过 GMA 治疗后，Bacteroides 的丰度降低，而 Faecalibacterium、Roseburia 和 Firmicutes 的丰度增加。
活动期 UC 与肠道微生物失调有关。GMA 治疗对 UC 患者的肠道微生物群具有强烈的调节作用。
