Laboratorio de Farmacognosia, UBIPRO Facultad de Estudios Superiores Iztacala UNAM, Tlalnepantla, Mexico.
Laboratorio de Farmacologia, Departamento de Ciencias de la Salud, DCBS, Universidad Autonoma Metropolitana, Iztapalapa, CDMX, Mexico.
J Evid Based Integr Med. 2021 Jan-Dec;26:2515690X20986762. doi: 10.1177/2515690X20986762.
is an endemic species of Mexico. Its latex is used to treat wounds, scarring, oral infections, and loose teeth. To date, there are no studies that validate at a morphological level a wound-healing use in diabetes. The present research aimed to evaluate the wound-healing capacity of the latex of in the skin of healthy and streptozotocin-induced diabetic mice. Also, a chemical analysis of the latex through molecular exclusion chromatography and HPLC were performed. Male mice () of 7-week-old CD1 strain were used. Groups of healthy and diabetic mice were formed. A longitudinal cut of 1 cm was performed on the depilated skin. All treatments were topically applied to the wound area twice a day for ten days. At the end of the experiments, the skin sections were obtained from the wound area and stained with Hematoxylin-Eosin. Then we counted the number of active fibroblasts in all the experimental groups. In normal mice, the latex accelerated the wound-healing process and decreased the number of active fibroblasts, similarly to Recoveron. In diabetic mice, the latex and Recoveron increased the number of active fibroblasts. In normal and diabetic mice, a thin and orderly epidermis was observed. Molecular exclusion chromatography exhibited 58 fractions, 14 of which were subjected to HPLC, to detect catechin, a flavonoid with antioxidant, antimicrobial, and anti-inflammatory properties. latex can be useful for wound treatment in patients with diabetes mellitus because it accelerates and promotes the wound-healing process.
是墨西哥的特有物种。它的乳胶被用于治疗创伤、疤痕、口腔感染和松动的牙齿。迄今为止，还没有研究从形态学水平验证其在糖尿病中的伤口愈合作用。本研究旨在评估 乳胶在健康和链脲佐菌素诱导的糖尿病小鼠皮肤中的伤口愈合能力。此外，还通过分子排阻色谱法和高效液相色谱法对乳胶进行了化学分析。使用 7 周龄 CD1 品系雄性小鼠（）。形成健康和糖尿病小鼠组。在去毛的皮肤上进行 1 厘米的纵向切口。所有治疗均在伤口区域每天两次局部应用 10 天。实验结束时，从伤口区域获得皮肤切片，并用苏木精-伊红染色。然后我们计算了所有实验组中活跃成纤维细胞的数量。在正常小鼠中，乳胶加速了伤口愈合过程并减少了活跃成纤维细胞的数量，与 Recoveron 相似。在糖尿病小鼠中，乳胶和 Recoveron 增加了活跃成纤维细胞的数量。在正常和糖尿病小鼠中，观察到薄而有序的表皮。分子排阻色谱法显示有 58 个馏分，其中 14 个馏分进行了高效液相色谱分析，以检测具有抗氧化、抗菌和抗炎特性的类黄酮儿茶素。 乳胶可用于治疗糖尿病患者的伤口，因为它可以加速和促进伤口愈合过程。
