Salim Muhammad Nur, Masyitha Dian, Harris Abdul, Balqis Ummu, Iskandar Cut Dahlia, Hambal Muhammad
Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
Laboratory of Histology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
Vet World. 2018 Feb;11(2):99-103. doi: 10.14202/vetworld.2018.99-103. Epub 2018 Feb 2.
The purpose of the present study was to determine the potential of latex in the cream formulation on CD68 immune expression (macrophages) during inflammatory phase wound healing process in mice skin.
Amount of 12 two-months-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. The mice were allotted into four groups of each, entire surface of each group wound covered by base cream control, sulfadiazine 0.1% cream, latex cream 10% and, 15%, respectively. All experiments were performed twice a day for 3 days. The wound healing was assayed in stained histological sections in immunohistochemical of the wounds. CD68 expression was investigated under a microscope.
The results showed that the cream from the 10% and 15% latex of revealed moderate immune reaction to CD68 on wound healing.
We concluded that the latex cream of possesses anti-inflammatory activity in wound healing process of mice skin.
本研究旨在确定乳膏制剂中的乳胶在小鼠皮肤炎症期伤口愈合过程中对CD68免疫表达（巨噬细胞）的影响。
选用12只2月龄、体重30至40克的雄性小鼠。进行手术操作，在每只动物的椎旁作2.0厘米长的伤口皮肤切口直至皮下。治疗在普鲁卡因乳膏局部麻醉下进行。将小鼠分为四组，每组伤口的整个表面分别用基础乳膏对照、0.1%磺胺嘧啶乳膏、10%乳胶乳膏和15%乳胶乳膏覆盖。所有实验每天进行两次，共3天。通过伤口免疫组织化学染色切片检测伤口愈合情况。在显微镜下观察CD68表达。
结果表明，10%和15%乳胶制成的乳膏在伤口愈合时对CD68显示出中度免疫反应。
我们得出结论，该乳胶乳膏在小鼠皮肤伤口愈合过程中具有抗炎活性。
