Balqis Ummu, Iskandar Cut Dahlia, Salim Muhammad Nur
Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
Laboratory of Microbiology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh 23111, Indonesia.
Vet World. 2018 Jul;11(7):939-943. doi: 10.14202/vetworld.2018.939-943. Epub 2018 Jul 15.
The aim of this research was to determine the angiogenesis activity of latex in cream formulation on CD34 immune expression during wound healing phase in mice skin.
Amount of 36 2-month-old male mice were used between 30 and 40 g. To surgical procedures, wound skin incision was performed 2.0 cm in length until subcutaneous on the paravertebral of each animal. The treatment was carried under locally anesthetized with procaine cream. All mice were divided into four groups, namely the base cream as control group (A), sulfadiazine 0.1% cream (B), latex cream 10% (C), and latex cream 15% (D). All groups were treated entire surface of wound. All experiments were performed twice a day for 10 days. Experiments were terminated on days 3, 7, and 10, respectively. The wound healing was assayed in stained histological section in immunohistochemical of the wounds. The CD34 expression was investigated under a microscope.
The results showed that the cream from 10% and 15% latex revealed moderate immune reaction to CD34 on days 3 and 7 in wound healing of mice skin.
We concluded that the cream from 10% and 15% latex has potential as angiogenesis activity in wound healing of mice skin.
本研究旨在确定乳膏制剂中的乳胶在小鼠皮肤伤口愈合阶段对CD34免疫表达的血管生成活性。
选用36只2月龄、体重30至40克的雄性小鼠。进行手术操作，在每只动物椎旁做一个2.0厘米长直至皮下的伤口皮肤切口。治疗在普鲁卡因乳膏局部麻醉下进行。所有小鼠分为四组，即作为对照组的基础乳膏（A组）、0.1%磺胺嘧啶乳膏（B组）、10%乳胶乳膏（C组）和15%乳胶乳膏（D组）。所有组均对伤口整个表面进行治疗。所有实验每天进行两次，共进行10天。分别在第3天、第7天和第10天终止实验。在伤口的免疫组织化学染色组织切片中检测伤口愈合情况。在显微镜下观察CD34表达。
结果表明，在小鼠皮肤伤口愈合的第3天和第7天，10%和15%乳胶制成的乳膏对CD34显示出中等程度的免疫反应。
我们得出结论，10%和15%乳胶制成的乳膏在小鼠皮肤伤口愈合中具有血管生成活性的潜力。
