Department of Hygiene and Epidemiology, Pomeranian Medical University, Szczecin, Poland.
Department of Interdisciplinary Dentistry, Pomeranian Medical University, Szczecin, Poland.
Oral Dis. 2022 Apr;28(3):771-776. doi: 10.1111/odi.13806. Epub 2021 Mar 1.
To examine the association of four FCN1 SNPs: -542G>A (rs10120023), -144C>A (rs10117466), +6658C>T (rs148649884), and +7895A>G (rs150625869) with dental caries in Polish children.
The study group consisted of 261 15-year-old Polish teenagers: 82 children with "higher" caries experience (having Decayed Missing Filled Teeth, DMFT >5) and 179 children with "lower" caries experience (having DMFT ≤5). Moreover, in additional comparison, a group of 229 children with caries experience (DMFT ≥1) was compared to a caries-free (DMFT =0) group of 32 children. Extraction of genomic DNA was performed from buccal swabs, and genotyping was performed by Real-Time PCR.
FCN1 SNPs +6658C>T and +7895A>G appeared to be monomorphic in our sample. The genotype, allele, or haplotype distributions in FCN1 SNPs -542G>A and -144C>A in children with "higher" caries experience did not differ significantly from those in "lower" caries experience group. Similar results with no significant differences were demonstrated for subjects with DMFT ≥1 compared to subjects with DMFT =0.
FCN1 SNPs are not the markers of dental caries susceptibility in Polish children.
研究 FCN1 的四个 SNP（-542G>A(rs10120023)、-144C>A(rs10117466)、+6658C>T(rs148649884)和+7895A>G(rs150625869)）与波兰儿童龋齿的关联性。
研究组由 261 名 15 岁的波兰青少年组成：82 名儿童有“更高”的龋齿经历（患有 Decayed Missing Filled Teeth，DMFT >5），179 名儿童有“更低”的龋齿经历（DMFT ≤5）。此外，在进一步的比较中，将一组有龋齿经历（DMFT ≥1）的 229 名儿童与无龋齿（DMFT =0）的 32 名儿童进行比较。从颊拭子中提取基因组 DNA，并通过 Real-Time PCR 进行基因分型。
FCN1 SNP +6658C>T 和 +7895A>G 在我们的样本中表现为单态性。在“更高”龋齿经历的儿童中，FCN1 SNP-542G>A 和 -144C>A 的基因型、等位基因或单倍型分布与“更低”龋齿经历的儿童无显著差异。DMFT ≥1 的受试者与 DMFT =0 的受试者相比，结果也相似，没有显著差异。
FCN1 SNP 不是波兰儿童龋齿易感性的标志物。
