Ohta Masumi, Nishimura Hiroyuki, Asada Yoshinobu
Arch Oral Biol. 2015 Jan;60(1):55-61. doi: 10.1016/j.archoralbio.2014.08.020.
In this study, we investigated whether single nucleotide polymorphisms (SNPs) in DLX3 are associated with dental caries susceptibility in Japanese children.
Genomic DNA of 201 Japanese children was extracted from buccal epithelial cells. The subjects were divided into two groups: 'low level' group with <10,000 colony forming units (CFU) of Streptococcus mutans/mL saliva (level 0) and 'high level' group with ≥ 10,000 CFU/mL (more than level 1). Each group was further divided according to decayed, missing, filled teeth (dmft) into low caries experience (dmft ≤2) and high caries experience (dmft ≥ 3). Seven SNPs in DLX3 were genotyped using TaqMan1® SNP Genotyping Assay.
Statistical significant association was observed between DLX3 (rs2278163) and caries experience in 'high level Mutans streptococci' group.
These findings suggest that rs2278163 SNP of DLX3 might be associated with dental caries susceptibility in Japanese children. T and C alleles of rs2278163 SNP may potentially be involved in caries susceptibility and caries protection respectively.
在本研究中，我们调查了DLX3基因中的单核苷酸多态性（SNP）是否与日本儿童的龋齿易感性相关。
从201名日本儿童的颊黏膜上皮细胞中提取基因组DNA。受试者被分为两组：变形链球菌/毫升唾液菌落形成单位（CFU）<10,000的“低水平”组（0级）和CFU≥10,000的“高水平”组（超过1级）。每组再根据龋失补牙数（dmft）分为低龋经验组（dmft≤2）和高龋经验组（dmft≥3）。使用TaqMan1®SNP基因分型检测法对DLX3中的7个SNP进行基因分型。
在“高水平变形链球菌”组中，观察到DLX3（rs2278163）与龋经验之间存在统计学显著关联。
这些发现表明，DLX3的rs2278163 SNP可能与日本儿童的龋齿易感性相关。rs2278163 SNP的T和C等位基因可能分别与龋齿易感性和龋齿保护有关。
