Mauviel A, Kähäri V M, Heino J, Daireaux M, Hartmann D J, Loyau G, Pujol J P
Laboratoire de Biochimie du Tissu Conjonctif, Université de Caen, CHU, France.
FEBS Lett. 1988 Apr 11;231(1):125-9. doi: 10.1016/0014-5793(88)80715-4.
The role of indomethacin in the regulation of extracellular matrix synthesis was studied in dermal fibroblast cultures. Indomethacin (10 microM) blocked totally the prostaglandin secretion and markedly increased the synthesis of collagen. In parallel, measurement of fibronectin, type I and type III procollagen mRNA levels showed a substantial increase under the action of indomethacin. On the other hand, indomethacin did not modify the mRNA levels of dermatan sulfate proteoglycan core protein. Measurement of collagen production estimated as the amount of collagenase digestible protein and by specific radioimmunoassay indicated a good correlation with the corresponding mRNA levels. These results suggest that indomethacin can regulate the extracellular matrix deposition at a transcriptional level.
在真皮成纤维细胞培养物中研究了吲哚美辛在细胞外基质合成调节中的作用。吲哚美辛（10微摩尔）完全阻断了前列腺素的分泌，并显著增加了胶原蛋白的合成。同时，对纤连蛋白、I型和III型前胶原mRNA水平的测量显示，在吲哚美辛的作用下有大幅增加。另一方面，吲哚美辛并未改变硫酸皮肤素蛋白聚糖核心蛋白的mRNA水平。以胶原酶可消化蛋白的量并通过特异性放射免疫测定法估计的胶原蛋白产量测量结果表明，其与相应的mRNA水平具有良好的相关性。这些结果表明，吲哚美辛可在转录水平调节细胞外基质的沉积。
