Master Program in Science and Health, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil.
Master Program in Science and Health, Universidade Federal do Piauí, Campus Ministro Petrônio Portella, CEP: 64059-550, Teresina, Piauí, Brazil.
Arch Oral Biol. 2021 Apr;124:105077. doi: 10.1016/j.archoralbio.2021.105077. Epub 2021 Feb 11.
The objective of this study was to perform a histopathological, morphometric and proteomic study of the parotid gland of dogs naturally infected with Leishmania infantum treated and not treated with Allopurinol.
Parotid glands from 14 dogs were used, divided into two groups: untreated and treated with oral allopurinol (20 mg / kg, once daily for 90 days). After adequate dissection, the organs were submitted to histopathological, histomorphometric and immunohistochemical techniques, using the monoclonal anti-β-catenin antibody.
Histopathological evaluation of treated and untreated groups showed acinar hypertrophy, structural disorganization of the nucleus and cytoplasm. There was an increase in the area and perimeter of the parotid acini in the experimental groups. The immunostaining of the β-catenin protein in the membrane was severely reduced in the treated and untreated groups.
These findings suggest that Leishmania infantum infection and treatment with Allopurinol alter the tissue structure of the parotid gland in dogs, promoting an increase in the acinar volume and a decrease in the expression of β-catenin in cell membranes.
本研究旨在对自然感染利什曼原虫的犬的腮腺进行组织病理学、形态计量学和蛋白质组学研究，并比较未经治疗和用别嘌醇治疗的犬的腮腺。
本研究使用了 14 只狗的腮腺，分为两组：未经治疗组和用别嘌醇（20mg/kg，每日一次，共 90 天）治疗组。充分解剖后，用抗β-连环蛋白单克隆抗体对器官进行组织病理学、组织形态计量学和免疫组织化学技术检测。
治疗组和未治疗组的组织病理学评价均显示腺泡肥大，核和细胞质结构紊乱。实验组的腮腺面积和周长增加。β-连环蛋白蛋白在膜中的免疫染色在治疗组和未治疗组中均严重减少。
这些发现表明，利什曼原虫感染和用别嘌醇治疗会改变犬腮腺的组织结构，导致腺泡体积增加，细胞膜中β-连环蛋白的表达减少。
