School of Pharmacy, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang 050017, People's Republic of China.
School of Pharmacy, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang 050017, People's Republic of China.
Food Chem. 2021 May 30;345:128560. doi: 10.1016/j.foodchem.2020.128560. Epub 2020 Nov 7.
An efficient and convenient detection method for organophosphorus pesticide (OP) residues is needed because of their high neurotoxicity and severe threat to food safety. OPs effectively reduce the production of thiocholine in the acetylcholinesterase/acetylthiocholine reaction by inhibiting the activity of acetylcholinesterase. Therefore, we developed a feasible and convenient fluorescent and colorimetric dual-response sensor based on the competitive complexation of Cu between graphitic carbon nitride nanosheets and thiocholine for the rapid detection of OPs with high sensitivity. Malathion was used as a model OP, and a linear range of 70-800 nM with a detection limit of 6.798 nM for a fluorescent signaling platform and 2.5-25 nM with a detection limit of 1.204 nM for a colorimetric probe were attained. The constructed probe was successfully applied to determine OP in actual samples of cabbages leaves and tap water. The results indicated that the dual-response probe was reliable and sensitive to actual samples.
由于有机磷农药 (OP) 具有高神经毒性和严重的食品安全威胁，因此需要一种高效、便捷的检测方法。OP 通过抑制乙酰胆碱酯酶的活性，有效减少乙酰胆碱酯酶/乙酰硫代胆碱反应中硫代胆碱的产生。因此，我们开发了一种基于石墨相氮化碳纳米片与硫代胆碱之间 Cu 的竞争络合作用的荧光和比色双响应传感器，用于快速检测 OP，具有高灵敏度。马拉硫磷被用作模型 OP，荧光信号平台的线性范围为 70-800 nM，检测限为 6.798 nM，比色探针的线性范围为 2.5-25 nM，检测限为 1.204 nM。构建的探针成功应用于白菜叶和自来水中 OP 的测定。结果表明，双响应探针对实际样品可靠且敏感。
