FeCo-NC 双原子纳米酶的优越过氧化物酶模拟活性用于智能手机基于视觉比色法测定有机磷农药。
Superior oxidase-mimetic activity of FeCo-NC dual-atom nanozyme for smartphone-based visually colorimetric assay of organophosphorus pesticides.
机构信息
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.
Jiangsu Key Laboratory of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, China.
出版信息
Mikrochim Acta. 2024 Jun 4;191(7):368. doi: 10.1007/s00604-024-06443-9.
A colorimetric analysis platform has been successfully developed based on FeCo-NC dual-atom nanozyme (FeCo-NC DAzyme) for the detection of organophosphorus pesticides (OPPs). The FeCo-NC DAzyme exhibited exceptional oxidase-like activity (OXD), enabling the catalysis of colorless TMB to form blue oxidized TMB (oxTMB) without the need for HO involvement. By combining acid phosphatase (ACP) hydrolase with FeCo-NC DAzyme, a "FeCo-NC DAzyme + TMB + ACP + SAP" colorimetric system was constructed, which facilitated the rapid detection of malathion. The chromogenic system was applied to detect malathion using a smartphone-based app and an auxiliary imaging interferogram device for colorimetric measurements, which have a linear range of 0.05-4.0 µM and a limit of detection (LOD) as low as 15 nM in real samples, comparable to UV-Vis and HPLC-DAD detection methods. Overall, these findings present a novel approach for convenient, rapid, and on-site monitoring of OPPs.
基于 FeCo-NC 双原子纳米酶（FeCo-NC DAzyme）成功开发了一种比色分析平台，用于检测有机磷农药（OPPs）。FeCo-NC DAzyme 表现出优异的过氧化物酶样活性（OXD），能够催化无色 TMB 形成蓝色氧化 TMB（oxTMB），而无需涉及 HO。通过将酸性磷酸酶（ACP）水解酶与 FeCo-NC DAzyme 结合，构建了“FeCo-NC DAzyme + TMB + ACP + SAP”比色系统，该系统可用于快速检测马拉硫磷。比色系统应用于智能手机应用程序和辅助成像干涉仪设备上的马拉硫磷检测，在实际样品中具有 0.05-4.0 μM 的线性范围和低至 15 nM 的检测限（LOD），与 UV-Vis 和 HPLC-DAD 检测方法相当。总的来说，这些发现为 OPPs 的方便、快速和现场监测提供了一种新方法。
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