Musa S N, Idris S, Lee Y L, Sekawi Z
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang Selangor, Malaysia.
Pathology Department, Sungai Buloh Hospital, 47000 Sungai Buloh, Selangor, Malaysia.
Trop Biomed. 2018 Jun 1;35(2):571-579.
Human adenovirus (HAdV) is one of the common pathogens that are responsible for a wide variety of infectious diseases. There are about 54 different adenovirus serotypes that are responsible for respiratory infections in humans. The prevalence of lower respiratory tract infection (LRTI) - associated with HAdV varies throughout different regions. The prevalence of HAdV in Malaysia is rarely investigated and reported despite severity of infection worldwide. This study was undertaken to identify the HAdV types associated with lower respiratory tract infection (LRTI) in Hospital Sungai Buloh, Malaysia between April 2013 until January 2014, a total of 210 specimens were collected from patients hospitalized with LRTI. Human adenovirus was detected using polymerase chain reaction (PCR). The positive products were sequenced and phylogenetic analysis of the virus was performed. Eighteen of 210 specimens (8.57%) were positive with HAdV infection. Based on the phylogenetic analysis study, HAdV-7 strains were the most common serotype with 11 cases, followed by HAdV-1, HAdV-2 and HAdV-4 with 2 cases each and one case of HAdV-5. The HAdV strains in this study were closely related to strains in Singapore and India. In this study, HAdV infection from LRTI patients in Hospital Sungai Buloh Malaysia were caused by different types of adenovirus mainly HAdV-7. This study will become a reference for further epidemiological study in this country.
人腺病毒（HAdV）是导致多种传染病的常见病原体之一。约有54种不同的腺病毒血清型可引起人类呼吸道感染。与HAdV相关的下呼吸道感染（LRTI）在不同地区的患病率有所不同。尽管全球感染情况严重，但马来西亚HAdV的患病率却鲜有调查和报道。本研究旨在确定2013年4月至2014年1月期间马来西亚双溪毛糯医院与下呼吸道感染（LRTI）相关的HAdV类型，共从LRTI住院患者中收集了210份标本。使用聚合酶链反应（PCR）检测人腺病毒。对阳性产物进行测序并对病毒进行系统发育分析。210份标本中有18份（8.57%）HAdV感染呈阳性。基于系统发育分析研究，HAdV - 7毒株是最常见的血清型，有11例，其次是HAdV - 1、HAdV - 2和HAdV - 4各2例，以及1例HAdV - 5。本研究中的HAdV毒株与新加坡和印度的毒株密切相关。在本研究中，马来西亚双溪毛糯医院LRTI患者的HAdV感染主要由不同类型的腺病毒引起，主要是HAdV - 7。本研究将为该国进一步的流行病学研究提供参考。
