Kalantari N, Sheikhansari M R, Ghaffari S, Alipour J, Gorgani-Firouzjaee T, Tamadoni A, Bayani M
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R Iran.
Student Research Committee, Babol University of Medical Sciences, Babol, I.R Iran.
Trop Biomed. 2018 Dec 1;35(4):1017-1027.
T. gondii is a life-threatening infection in immunocompromised patients which may be transmitted through blood transfusion. The present study aimed to evaluate the seroprevalence and molecular detection of T. gondii infection and the associated risk factors among young healthy blood donors in the central part of Mazandaran province, northern Iran. Blood samples were taken from 500 participants and the serum was separated. All serum samples were tested for the presence of anti-T. gondii antibodies (IgG) and then all positive samples were evaluated for IgM antibodies using commercial ELISA kits. All IgM positive samples and 66 randomly selected IgG positive samples were further tested by PCR of the REP-529 gene. Anti-Toxoplasma antibodies (IgG) avidity test was performed for 142 IgG positive samples which were randomly selected. In the current study, anti-T. gondii antibodies (IgG) and (IgM) were found in 316 (63.2%) and 3 (0.95 %) participants, respectively. Seropositivity rate of Toxoplasma was higher among blood donors living in rural areas (P=0.000) and those with a history of soil and animal contact (P<0.05). PCR of the REP-529 gene showed T. gondii DNA in 21 out of 66 samples. The REP-529 gene was not detected in IgM positive samples. Low avidity antibodies (IgG) was found in 23.2% of the IgG positive samples. In conclusions, this study found that the prevalence of toxoplasmosis among young healthy blood donors in north of Iran was high. To reduce the risk of parasite transmission, leukofilteration method are recommended for donated blood used for immunosuppressed patients.
弓形虫对免疫功能低下的患者来说是一种危及生命的感染，可通过输血传播。本研究旨在评估伊朗北部马赞德兰省中部年轻健康献血者中弓形虫感染的血清流行率、分子检测情况及相关危险因素。采集了500名参与者的血样并分离出血清。所有血清样本均检测抗弓形虫抗体（IgG），然后使用商用ELISA试剂盒对所有阳性样本检测IgM抗体。所有IgM阳性样本和66个随机选择的IgG阳性样本通过REP - 529基因的PCR进一步检测。对随机选择的142个IgG阳性样本进行抗弓形虫抗体（IgG）亲和力检测。在本研究中，分别在316名（63.2%）和3名（0.95%）参与者中发现了抗弓形虫抗体（IgG）和（IgM）。生活在农村地区的献血者以及有土壤和动物接触史的献血者中弓形虫血清阳性率更高（P = 0.000）和（P < 0.05）。REP - 529基因的PCR显示66个样本中有21个存在弓形虫DNA。在IgM阳性样本中未检测到REP - 529基因。在23.2%的IgG阳性样本中发现低亲和力抗体（IgG）。总之，本研究发现伊朗北部年轻健康献血者中弓形虫病的患病率很高。为降低寄生虫传播风险，建议对用于免疫抑制患者的献血采用白细胞过滤法。
