Sadooghian Saeed, Mahmoudvand Hossein, Mohammadi Mohammad Ali, Nazari Sarcheshmeh Naser, Tavakoli Kareshk Amir, Kamiabi Hossein, Zia-Ali Naser
Dept. of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran.
Dept. of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran.
Iran J Parasitol. 2017 Oct-Dec;12(4):554-562.
This cross-sectional investigation aimed to evaluate the prevalence of IgM and IgG anti- antibodies and the associated risk factors among healthy blood donors in Khorasan Razavi Province, northeast of Iran from Nov 2014 to May 2015.
Overall, 491 serum samples from apparently healthy blood donors referred the six biggest blood centers in Razavi Khorasan, Iran, were screened for IgG and IgM anti- antibodies by enzyme-linked immunosorbent assay (ELISA). A structured questionnaire was used to obtain information on risk factors for infection. Nested PCR was also used to detect DNA of in the IgM-positive samples by using of B1 and RE (Repetitive Element) as marker for amplifying fragment size of 531 bp and 164 bp in PCR method.
Totally, 200 (40.7%) samples were seropositive for anti- antibodies; 184 (37.5%) donors tested seropositive for only IgG antibody, 8 (1.6%) tested seropositive for both IgM and IgG and 8 (1.6%) were positive for IgM antibody alone. Several risk factors significantly related to seropositivity in the univariate analysis at <0.05 included age (<0.001), and raw/half-cocked meat consumption (=0.015). DNA was found in all sixteen IgM-positive samples.
infection was present among healthy blood donors in northeast of Iran. Thus, it is suggested to design screening programs for preventing transfusion-transmitted toxoplasmosis.
本横断面调查旨在评估2014年11月至2015年5月间伊朗东北部呼罗珊拉扎维省健康献血者中抗[病原体名称] IgM和IgG抗体的流行率及其相关危险因素。
总共对来自伊朗呼罗珊拉扎维最大的六个血液中心的491份看似健康的献血者血清样本进行酶联免疫吸附测定（ELISA），以筛查IgG和IgM抗[病原体名称]抗体。使用结构化问卷获取感染危险因素的信息。还采用巢式聚合酶链反应（Nested PCR），以B1和重复元件（RE）为标记，通过PCR方法扩增531 bp和164 bp的片段大小，检测IgM阳性样本中的[病原体名称] DNA。
总共有200份（40.7%）样本抗[病原体名称]抗体血清学呈阳性；184名（37.5%）献血者仅IgG抗体血清学呈阳性，8名（1.6%）献血者IgM和IgG均呈阳性，8名（1.6%）仅IgM抗体呈阳性。在单因素分析中，几个危险因素与抗[病原体名称]血清学阳性显著相关（P<0.05），包括年龄（P<0.001）和食用生肉/半熟肉（P=0.015）。在所有16份IgM阳性样本中均发现了[病原体名称] DNA。
伊朗东北部的健康献血者中存在[病原体名称]感染。因此，建议设计筛查方案以预防输血传播的弓形虫病。
