Kimura M, Kimura I, Nakamura T, Nojima H
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Diabetologia. 1988 Feb;31(2):103-7. doi: 10.1007/BF00395556.
The electrical properties of skeletal muscle membranes were investigated in genetically diabetic KK-CAy mice and alloxan-induced diabetic ddY mice. Using isolated phrenic nerve-diaphragm muscle or sciatic nerve-gastrocnemius muscle in situ preparations, nerve-stimulated twitch tensions (the maximal value) were obtained at lower voltage pulse in diabetic KK-CAy mice than in normal ddY mice. The diabetic state reduced resting membrane potentials (1.7-4.0 mV) and resting membrane conductance (0.37-0.44 mu siemen), decreased the amplitude (3.8-3.9 mV) and overshoot (4.5 mV) of directly induced-action potential, and prolonged action potential duration. In the diabetic state, resting membrane conductance was multiply-correlated with blood glucose level and resting membrane potential. In alloxan-induced diabetic mice, resting membrane potentials were significantly multiply-correlated with the weeks elapsed after alloxan injection and blood glucose level (p less than 0.01). Since the reduction of resting membrane potential correlated with the weeks, changes in resting membrane potential may be involved in the decrease in insulin-like growth factor action. The reduction of resting membrane conductance was correlated with the increase in blood glucose.
在遗传性糖尿病KK-CAy小鼠和四氧嘧啶诱导的糖尿病ddY小鼠中研究了骨骼肌膜的电特性。使用分离的膈神经-膈肌或坐骨神经-腓肠肌原位标本，在糖尿病KK-CAy小鼠中，较低电压脉冲下获得的神经刺激抽搐张力（最大值）低于正常ddY小鼠。糖尿病状态降低了静息膜电位（1.7 - 4.0 mV）和静息膜电导（0.37 - 0.44微西门子），降低了直接诱导动作电位的幅度（3.8 - 3.9 mV）和超射（4.5 mV），并延长了动作电位持续时间。在糖尿病状态下，静息膜电导与血糖水平和静息膜电位呈多重相关。在四氧嘧啶诱导的糖尿病小鼠中，静息膜电位与四氧嘧啶注射后经过的周数和血糖水平显著多重相关（p < 0.01）。由于静息膜电位的降低与周数相关，静息膜电位的变化可能与胰岛素样生长因子作用的降低有关。静息膜电导的降低与血糖升高相关。
