Grossie J
Diabetes. 1982 Mar;31(3):194-202. doi: 10.2337/diab.31.3.194.
Diabetes mellitus was produced by withholding insulin from female rats previously treated with alloxan. In vitro studies of contractile and electrical properties of the extensor digitorum longus muscle were analyzed at various periods following cessation of insulin treatment. In severely diabetic ketoacidotic rat muscles it was observed that both direct and indirectly induced twitch and tetanic tensions were significantly reduced to the same degree. Resting membrane potentials were significantly reduced but repolarized when external chloride was removed. Indirectly induced action potential amplitude and rate of depolarization were significantly depressed and there was a significant increase in action potential duration. Specific membrane in action potential duration. Specific membrane resistance decreased significantly but transiently due largely to an increase in chloride conductance and specific capacitance increased significantly. After extended periods of milder diabetes, specific tetanic tension decreased slightly while specific twitch tension was unchanged in both direct and indirectly induced contractions. Resting potentials were only slightly reduced. Indirectly elicited action potential amplitude and rate of depolarization were slightly reduced. Specific membrane resistance was significantly increased largely due to decreases in chloride conductance. Specific capacitance was unchanged.
通过对先前用四氧嘧啶处理过的雌性大鼠停止注射胰岛素来诱发糖尿病。在停止胰岛素治疗后的不同时间段，对趾长伸肌的收缩和电特性进行了体外研究。在严重糖尿病酮症酸中毒大鼠的肌肉中观察到，直接和间接诱发的抽搐和强直张力均显著降低至相同程度。静息膜电位显著降低，但去除细胞外氯离子后可复极化。间接诱发的动作电位幅度和去极化速率显著降低，动作电位持续时间显著增加。动作电位持续期间的比膜电阻显著但短暂降低，这主要是由于氯离子电导增加，比电容显著增加。在轻度糖尿病持续较长时间后，强直比张力略有下降，而直接和间接诱发收缩中的抽搐比张力均未改变。静息电位仅略有降低。间接诱发的动作电位幅度和去极化速率略有降低。比膜电阻显著增加，这主要是由于氯离子电导降低。比电容未改变。
