Morphophysiology Department, Faculty of Agricultural Sciences, University of Camagüey Ignacio Agramonte y Loynaz, 74 650, Camagüey, Cuba.
CERAD CoE, Department of Paraclinical Sciences, Norwegian University of Life Sciences, P.O. Box 5003, Ås, Norway.
Sci Rep. 2021 Feb 18;11(1):4142. doi: 10.1038/s41598-021-83345-3.
Gamma radiation produces DNA instability and impaired phenotype. Previously, we observed negative effects on phenotype, DNA methylation, and gene expression profiles, in offspring of zebrafish exposed to gamma radiation during gametogenesis. We hypothesize that previously observed effects are accompanied with changes in the expression profile of non-coding RNAs, inherited by next generations. Non-coding RNA expression profile was analysed in F offspring (5.5 h post-fertilization) by high-throughput sequencing 1 year after parental irradiation (8.7 mGy/h, 5.2 Gy total dose). Using our previous F-γ genome-wide gene expression data (GSE98539), hundreds of mRNAs were predicted as targets of differentially expressed (DE) miRNAs, involved in pathways such as insulin receptor, NFkB and PTEN signalling, linking to apoptosis and cancer. snRNAs belonging to the five major spliceosomal snRNAs were down-regulated in the F-γ group, Indicating transcriptional and post-transcriptional alterations. In addition, DEpiRNA clusters were associated to 9 transposable elements (TEs) (LTR, LINE, and TIR) (p = 0.0024), probable as a response to the activation of these TEs. Moreover, the expression of the lincRNAs malat-1, and several others was altered in the offspring F, in concordance with previously observed phenotypical alterations. In conclusion, our results demonstrate diverse gamma radiation-induced alterations in the ncRNA profiles of F offspring observable 1 year after parental irradiation.
γ 射线会导致 DNA 不稳定和表型损伤。此前，我们观察到在配子发生期接受 γ 射线照射的斑马鱼后代的表型、DNA 甲基化和基因表达谱出现负向变化。我们假设，之前观察到的效应伴随着下一代非编码 RNA 表达谱的变化。通过高通量测序，在亲代辐照后 1 年（8.7mGy/h，总剂量 5.2Gy）分析了 F1 代（受精后 5.5 小时）的非编码 RNA 表达谱。利用我们之前的 F-γ 全基因组基因表达数据（GSE98539），预测了数百个 mRNA 是差异表达 miRNA 的靶标，这些 miRNA 参与了胰岛素受体、NFkB 和 PTEN 信号通路等途径，与细胞凋亡和癌症有关。属于五个主要剪接体 snRNA 的 snRNAs 在 F-γ 组中下调，表明存在转录和转录后改变。此外，差异表达 piRNA 簇与 9 个转座元件（LTR、LINE 和 TIR）（p=0.0024）相关，可能是这些转座元件被激活的反应。此外，lincRNA malat-1 和其他几种 lincRNA 的表达在后代 F 中发生改变，与之前观察到的表型改变一致。总之，我们的研究结果表明，γ 射线诱导的 F1 代非编码 RNA 谱发生了多种变化，这些变化在亲代辐照后 1 年即可观察到。
