Gu Juan, Zhang Shu, He Xin, Chen Sufang, Wang Yan
Department of Public Health, Jiangsu Vocational College of Medicine, Yancheng, Jiangsu 224005, P.R. China.
Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
Exp Ther Med. 2021 Mar;21(3):249. doi: 10.3892/etm.2021.9680. Epub 2021 Jan 22.
P53-induced gene 11 (PIG11) is an early transcription-related target of p53 that is involved in cell apoptosis and tumor development. However, its biological function in gastric cancer (GC) tissues and relationship with the prognosis of patients with GC have remained elusive. In the present retrospective study, 60 fresh and 790 paraffin-embedded samples of GC were obtained from the Affiliated Hospital of Nantong University (Nantong, China) with complete clinical data from all patients. Reverse transcription-quantitative PCR and tissue microarray-immunohistochemical analysis were used to determine the expression of PIG11 in the respective GC tissues. A receiver operating characteristic (ROC) curve was plotted to determine the diagnostic utility of PIG11 expression in GC. Furthermore, three online databases, including Gene Expression Profiling Interactive Analysis (GEPIA), Oncomine and Kaplan-Meier plotter, were used for bioinformatics analysis of PIG11. PIG11 expression in GC tissues was high, which was positively correlated with invasive depth (P<0.001), lymph node metastasis (P<0.001), distant metastasis (P=0.019), TNM staging (P<0.001) and carcinoembryonic antigen in serum (P<0.001), and negatively associated with the overall survival of patients with GC. The ROC curve analysis suggested that based on PIG11 expression, it was possible to distinguish GC tissues from adjacent normal tissues (P<0.0001) with a sensitivity and specificity of 81.67 and 76.67%, respectively. In addition, Cox logistic regression analysis demonstrated that high PIG11 expression is a novel biomarker for unfavorable prognosis of patients with GC. Furthermore, the results obtained from the GEPIA database indicated that PIG11 expression is correlated with TNF, carcinoembryonic antigen related cell adhesion molecule 5, phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit alpha, VEGFA and kinase insert domain receptor. Therefore, PIG11 expression may be associated with the malignancy of GC and may serve as a potential diagnostic and prognostic biomarker for GC.
p53诱导基因11（PIG11）是p53的一个早期转录相关靶点，参与细胞凋亡和肿瘤发展。然而，其在胃癌（GC）组织中的生物学功能以及与GC患者预后的关系仍不清楚。在本回顾性研究中，从南通大学附属医院（中国南通）获取了60份新鲜的和790份石蜡包埋的GC样本，并获得了所有患者完整的临床数据。采用逆转录定量PCR和组织芯片免疫组化分析来确定PIG11在相应GC组织中的表达。绘制受试者工作特征（ROC）曲线以确定PIG11表达在GC中的诊断效用。此外，利用包括基因表达谱交互式分析（GEPIA）、Oncomine和Kaplan-Meier plotter在内的三个在线数据库对PIG11进行生物信息学分析。GC组织中PIG11表达较高，与浸润深度（P<0.001）、淋巴结转移（P<0.001）、远处转移（P=0.019）、TNM分期（P<0.001）和血清癌胚抗原（P<0.001）呈正相关，与GC患者的总生存期呈负相关。ROC曲线分析表明，基于PIG11表达，可以将GC组织与相邻正常组织区分开来（P<0.0001），敏感性和特异性分别为81.67%和76.67%。此外，Cox逻辑回归分析表明，PIG11高表达是GC患者预后不良的一个新的生物标志物。此外，从GEPIA数据库获得的结果表明，PIG11表达与肿瘤坏死因子、癌胚抗原相关细胞粘附分子5、磷脂酰肌醇-4,5-二磷酸3-激酶催化亚基α、血管内皮生长因子A和激酶插入结构域受体相关。因此，PIG11表达可能与GC的恶性程度有关，并可能作为GC潜在的诊断和预后生物标志物。
