Sun Xuan, Zhang Hong-Fei, Ma Chao-Lin, Wei Hua, Li Bao-Ming, Luo Jie
School of Life Sciences, Nanchang University, Nanchang 330031, China.
Institute of Life Science, Nanchang University, Nanchang 330031, China.
Can J Infect Dis Med Microbiol. 2021 Jan 30;2021:6613903. doi: 10.1155/2021/6613903. eCollection 2021.
Intestinal microorganisms play an important role in regulating the neurodevelopment and the brain functions of the host through the gut-brain axis. , one of the most representative intestinal probiotics, produces important effects on human physiological functions. Our previous studies reveal that the WLPL04 has a series of beneficial actions, such as antiadhesion of pathogens, protection from the harmful effect of sodium dodecyl sulfate, and anti-inflammatory stress on Caco2 cells. However, its effects on brain functions remain unknown. The present study aims to evaluate the potential effect of WLPL04 on anxiety/depressive-like behaviors in chronically restrained mice.
Newly weaned mice were exposed to chronic restraint stress for four weeks and raised daily with or without . WLPL04 water supplement. Animals were behaviorally assessed for anxiety/depression and cognitive functions. The 16S rRNA sequencing was performed to analyze the intestinal microbiota structure. The levels of the medial prefrontal cortical (mPFC) brain-derived neurotrophic factor (BDNF)/tropomyosin-related kinase B (TrkB) and serum 5-hydroxytryptamine (5-HT) were examined using Western blot and enzyme-linked immunosorbent assay.
The chronic stress-induced anxiety/depressive-like behaviors and cognitive deficits were significantly alleviated by the . WLPL04 treatment. The 16S rRNA sequencing analysis showed that the chronic stress reduced the diversity and the richness of intestinal microbiota, which were rescued by the . WLPL04 treatment. The levels of BDNF and TrkB in the mPFC and the concentration of 5-HT in the serum remained unchanged in chronically restrained mice treated with the WLPL04.
The WLPL04 can rescue anxiety/depressive-like behaviors and cognitive dysfunctions, reverse the abnormal change in intestinal microbiota, and alleviate the reduced levels of 5-HT, BDNF, and TrkB induced by chronic stress in mice, providing an experimental basis for the therapeutic application of on anxiety/depression.
肠道微生物通过肠-脑轴在调节宿主神经发育和脑功能方面发挥重要作用。作为最具代表性的肠道益生菌之一，对人体生理功能产生重要影响。我们之前的研究表明，WLPL04具有一系列有益作用，如病原体抗黏附、抵御十二烷基硫酸钠的有害影响以及对Caco2细胞的抗炎应激作用。然而，其对脑功能的影响尚不清楚。本研究旨在评估WLPL04对慢性束缚小鼠焦虑/抑郁样行为的潜在影响。
将刚断奶的小鼠暴露于慢性束缚应激4周，并每日给予或不给予WLPL04水补充剂。对动物进行焦虑/抑郁和认知功能的行为评估。进行16S rRNA测序以分析肠道微生物群结构。使用蛋白质免疫印迹法和酶联免疫吸附测定法检测内侧前额叶皮质（mPFC）脑源性神经营养因子（BDNF）/原肌球蛋白相关激酶B（TrkB）水平和血清5-羟色胺（5-HT）水平。
WLPL04治疗显著减轻了慢性应激诱导的焦虑/抑郁样行为和认知缺陷。16S rRNA测序分析表明，慢性应激降低了肠道微生物群的多样性和丰富度，而WLPL04治疗可使其恢复。在接受WLPL04治疗的慢性束缚小鼠中，mPFC中BDNF和TrkB的水平以及血清中5-HT的浓度保持不变。
WLPL04可挽救焦虑/抑郁样行为和认知功能障碍，逆转肠道微生物群的异常变化，并减轻慢性应激诱导的小鼠5-HT、BDNF和TrkB水平降低，为其在焦虑/抑郁治疗中的应用提供了实验依据。
