Department of Pathogenic Biology, Chengdu Medical College, Chengdu, China.
Department of Clinical Laboratory, Pidu District People's Hospital, Chengdu, China.
PLoS One. 2022 Sep 9;17(9):e0274244. doi: 10.1371/journal.pone.0274244. eCollection 2022.
This study characterized the whole genome of Companilactobacillus futsaii subsp. chongqingii CQ16Z1 isolated from Chongqing of China, performed genome sequence analysis with Companilactobacillus futsaii subsp. futsaii YM0097 isolated from Taiwan of China, and experimentally verified drug resistance and effect on the exploratory behavior of male C57BL/6 mice and analysis of gut microbiota and metabolomic studies. The genome of CQ16Z1 is 2.6 Mb. Sequence analysis between genomes showed that the two strains are Companilactobacillus futsaii. The unique genes of CQ16Z1 and YM0097 are 217 and 267, which account for 9% and 11% of the whole genomes, respectively. According to unique gene annotation, the results showed that genes associated with carbohydrate metabolism, environmental information processing, metabolism of cofactors and vitamins, cell wall/membrane/envelope biogenesis, phage and drug resistance are significantly different. The results of the drug resistance experiment showed that YM0097 had different degrees of resistance to 13 antibiotics, while CQ16Z1 was sensitive to more than half of them. YM0097 contains 9 prophage regions and CQ16Z1 contains 3 prophage regions. The results of the open field test showed that the time (P = 0.005; P = 0.047) and distance (P < 0.010; P = 0.046) of the central area of Y97 group and CQ group are significantly different from the control group. The results of the elevated plus maze test showed that compared with the control group, Y97 group had significant differences in the number of entries to the open arms and the percentage of open arms entry times (P = 0.004; P = 0.025), while the difference between the CQ group and the control group was not significant. YM0097 has a more obvious effect on the exploratory behavior of mice. The effects of YM0097 and CQ16Z1 on the intestinal flora of mice are also different. YM0097 may be more beneficial to the intestinal flora of the host. And LC/MS also showed that the metabolic effects of the two strains on the host are different. Finally, we believe that YM0097 is more suitable for application research as a psychobiotics.
本研究对分离自中国重庆的Companilactobacillus futsaii 亚种 chongqingii CQ16Z1 进行了全基因组特征描述，并与分离自中国台湾的Companilactobacillus futsaii 亚种 futsaii YM0097 进行了基因组序列分析，同时还进行了药物抗性和对雄性 C57BL/6 小鼠探索行为的影响实验以及肠道微生物组和代谢组学研究。CQ16Z1 的基因组大小为 2.6 Mb。对两个基因组之间的序列分析表明，两株菌均为 Companilactobacillus futsaii。CQ16Z1 和 YM0097 的特有基因分别为 217 和 267 个，分别占整个基因组的 9%和 11%。根据特有基因注释，结果表明与碳水化合物代谢、环境信息处理、辅因子和维生素代谢、细胞壁/膜/包膜生物发生、噬菌体和药物抗性相关的基因有显著差异。药物抗性实验结果表明，YM0097 对 13 种抗生素有不同程度的抗性，而 CQ16Z1 对其中一半以上的抗生素敏感。YM0097 含有 9 个噬菌体区域，而 CQ16Z1 含有 3 个噬菌体区域。旷场实验结果表明，YM0097 组和 CQ 组的中央区域时间（P = 0.005；P = 0.047）和距离（P < 0.010；P = 0.046）与对照组有显著差异。高架十字迷宫实验结果表明，与对照组相比，YM0097 组进入开放臂的次数和进入开放臂的时间百分比有显著差异（P = 0.004；P = 0.025），而 CQ 组与对照组之间的差异不显著。YM0097 对小鼠的探索行为有更明显的影响。YM0097 和 CQ16Z1 对小鼠肠道菌群的影响也不同。YM0097 可能更有利于宿主的肠道菌群。LC/MS 还表明，两株菌对宿主的代谢作用不同。最后，我们认为 YM0097 更适合作为一种益生菌进行应用研究。
