Gao Kan, Chen Cailing, Zheng Zhiyao, Fan Qiuling, Wang Haifeng, Li Yanjun, Chen Su
Research and Development Department, Hangzhou Wahaha Group Co., Ltd., Hangzhou, China.
Hangzhou Wahaha Technology Co., Ltd., Hangzhou, China.
Front Nutr. 2024 Aug 1;11:1439094. doi: 10.3389/fnut.2024.1439094. eCollection 2024.
Aging often accompanies cognitive and mood disturbances. Emerging evidence indicates that specific probiotics mitigate cognitive and mood dysfunctions. Strains within , a subgroup of probiotics, including and are shown beneficial effects on brain functions via the gut microbiota-brain axis (GBA). Our previous study identified two strains ( WHH2078 and WHH2080) with the ability to promote the secretion of gut 5-hydroxytryptophan (5-HTP), the precursor of the GBA mediator 5-hydroxytryptamine (5-HT). In this study, the modulatory effects of WHH2078 and WHH2080 on cognitive and mood alternations were investigated in aged mice. Oral administration of WHH2078 and WHH2080 (1 × 10 CFU/mL/day) in aged mice (12-month-old) for 12 weeks significantly improved cognitive and depressive-and anxiety-like behaviors. The neuronal loss, the 5-HT metabolism dysfunction, and the neuroinflammation in the hippocampus of aged mice were restored by WHH2078 and WHH2080. the disturbances in the serum tryptophan metabolism in aged mice were unveiled by metabolomics, notably with decreased levels of 5-HT and 5-HTP, and increased levels of kynurenine, 3-hydroxykynurenine, and indolelactic acid, which were reversed by WHH2078 and WHH2080. Regarding the gut microbial community, WHH2078 and WHH2080 restored the increased abundance of , , and and the decreased abundance of and in aged mice. The beneficial effects of the two strains were linked to the modulation of 5-HT metabolism and gut microbiota. Our findings point to the potential role of strains with 5-HTP-promoting abilities as therapeutic approaches for age-related cognitive and mood disorders.
衰老常伴有认知和情绪障碍。新出现的证据表明，特定的益生菌可减轻认知和情绪功能障碍。益生菌的一个亚组中的菌株，包括[具体菌株名称未给出]，已显示出通过肠道微生物群-脑轴（GBA）对脑功能有有益作用。我们之前的研究鉴定出两株[具体菌株名称未给出]（WHH2078和WHH2080）具有促进肠道5-羟色氨酸（5-HTP）分泌的能力，5-HTP是GBA介质5-羟色胺（5-HT）的前体。在本研究中，研究了WHH2078和WHH2080对老年小鼠认知和情绪变化的调节作用。对12月龄老年小鼠口服WHH2078和WHH2080（1×10⁹CFU/mL/天），持续12周，显著改善了认知以及抑郁和焦虑样行为。WHH2078和WHH2080恢复了老年小鼠海马体中的神经元丢失、5-HT代谢功能障碍和神经炎症。代谢组学揭示了老年小鼠血清色氨酸代谢的紊乱，特别是5-HT和5-HTP水平降低，犬尿氨酸、3-羟基犬尿氨酸和吲哚乳酸水平升高，而WHH2078和WHH2080可使其逆转。关于肠道微生物群落，WHH2078和WHH2080恢复了老年小鼠中[具体微生物名称未给出]丰度的增加以及[具体微生物名称未给出]丰度的降低。这两种菌株的有益作用与5-HT代谢和肠道微生物群的调节有关。我们的研究结果表明，具有促进5-HTP能力的[具体菌株名称未给出]菌株作为与年龄相关的认知和情绪障碍的治疗方法具有潜在作用。
